Ciz1 cooperates with cyclin-A-CDK2 to activate mammalian DNA replication in vitro

• Published in: Journal of cell science Vol. 123; no. 7; pp. 1108 - 1115
• Main Authors: Copeland, Nikki A, Sercombe, Heather E, Ainscough, Justin F.X, Coverley, Dawn
• Format: Journal Article
• Language: English
• Published: England The Company of Biologists Limited 01.04.2010; Company of Biologists
• Subjects: Animals; BALB 3T3 Cells; Cell Nucleus - metabolism; Cell-Free System; Cloning, Molecular; Cyclin A - metabolism; Cyclin E - metabolism; Cyclin-Dependent Kinase 2 - metabolism; DNA Replication; HeLa Cells; Humans; Mice; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Protein Binding; RNA, Small Interfering - genetics

Initiation of mammalian DNA replication can be reconstituted from isolated G1-phase nuclei and cell extracts, supplemented with cyclin-dependent protein kinases (CDKs). Under these conditions, cyclin E supports pre-replication complex assembly, whereas cyclin-A-associated kinase acts later to termin...