How thick are the paraffin-embedded tissue sections routinely prepared in laboratory? A morphometric study using a confocal laser scanning microscope

• Published in: Pathology international Vol. 48; no. 3; pp. 179 - 183
• Main Authors: Masuda, Tomoyuki, Kawaguchi, Junzo, Oikawa, Hiroki, Yashima, Akiko, Suzuiki, Kazuyuki, Sato, Shun-ichi, Satodate, Ryoichl
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.1998
• Subjects: Animals; confocal laser scanning microscopy; Liver - anatomy & histology; Male; Mathematics; Microscopy, Confocal; Microtomy; Paraffin Embedding; paraffin-embedded tissue; Rats; Rats, Wistar; section thickness; stereology

In order to evaluate the differences between cut and measured thicknesses of formalin‐fixed, paraffin‐embedded tissue sections, formalin‐fixed, paraffin‐embedded blocks of liver tissue were prepared from four male Wlstar rats. The sections were cut at preselected thicknesses of 3, 6, 9,12, 15 and 18...