Spatial organization of transcribing loci during early genome activation in Drosophila

• Published in: Current biology Vol. 31; no. 22; pp. 5102 - 5110.e5
• Main Authors: Huang, Shao-Kuei, Whitney, Peter H., Dutta, Sayantan, Shvartsman, Stanislav Y., Rushlow, Christine A.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 22.11.2021
• Subjects: Animals; Drosophila - genetics; Drosophila - metabolism; Drosophila melanogaster - genetics; Drosophila Proteins - genetics; Drosophila Proteins - metabolism; Gene Expression Regulation, Developmental; genome activation; histone locus body; Nuclear Proteins - metabolism; RNA polymerase II; RNA Polymerase II - genetics; speckles; Transcription Factors - metabolism; Transcriptional Activation; Zelda; genome activation; histone locus body; speckles; Zelda; RNA polymerase II

The early Drosophila embryo provides unique experimental advantages for addressing fundamental questions of gene regulation at multiple levels of organization, from individual gene loci to the entire genome. Using 1.5-h-old Drosophila embryos undergoing the first wave of genome activation,1 we detec...