TNF-α suppresses dendritic cell death and the production of reactive oxygen intermediates induced by plasma withdrawal

• Published in: Experimental dermatology Vol. 13; no. 5; pp. 282 - 288
• Main Authors: Um, Hong-Duck, Cho, Young-Hun, Kim, Do Kyun, Shin, Jong-Ran, Lee, Yung-Jae, Choi, Kwang-Sung, Kang, Jin-Moon, Lee, Min-Geol
• Format: Journal Article
• Language: English
• Published: Oxford, UK; Malden, USA Munksgaard International Publishers 01.05.2004; Blackwell
• Subjects: Acetylcysteine - pharmacology; Antigens, CD - analysis; Antigens, CD - metabolism; antioxidants; Antioxidants - pharmacology; Biological and medical sciences; catalase; Catalase - pharmacology; Cell Death - drug effects; Cell Differentiation - drug effects; Cell Survival - drug effects; Culture Media - pharmacology; Cytokines - pharmacology; dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Dendritic Cells - physiology; Dermatology; Dinoprostone - pharmacology; Flow Cytometry; Glutathione - pharmacology; HLA-DR Antigens - analysis; HLA-DR Antigens - metabolism; Humans; Hydrogen Peroxide - pharmacology; Medical sciences; N-acetylcysteine; Plasma - physiology; reactive oxygen intermediates; Reactive Oxygen Species - analysis; Reactive Oxygen Species - metabolism; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - pharmacology; Human; Dendritic cell; Cell culture; Oxygen; Enzyme; Dermatology; antioxidants; Acetylcysteine; Antioxidant; reactive oxygen intermediates; Blood plasma; N-acetylcysteine; tumor necrosis factor-alpha; Mucolytic; Peroxidases; Catalase; Antigen presenting cell; Cell death; Production; dentritic cell; Oxidoreductases; Tumor necrosis factor α; Apoptosis

:  Mature dendritic cells (DCs) were generated by culturing human peripheral blood monocytes for 7 days and, then, treating them with a cytokine cocktail for 2 days. The viability of the mature DCs (Day 9) obtained was approximately 60–70%, and this gradually declined when they were recultured in X‐...