Rapid generation of potent antibodies by autonomous hypermutation in yeast

• Published in: Nature chemical biology Vol. 17; no. 10; pp. 1057 - 1064
• Main Authors: Wellner, Alon, McMahon, Conor, Gilman, Morgan S. A., Clements, Jonathan R., Clark, Sarah, Nguyen, Kianna M., Ho, Ming H., Hu, Vincent J., Shin, Jung-Eun, Feldman, Jared, Hauser, Blake M., Caradonna, Timothy M., Wingler, Laura M., Schmidt, Aaron G., Marks, Debora S., Abraham, Jonathan, Kruse, Andrew C., Liu, Chang C.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.10.2021; Nature Publishing Group
• Subjects: 631/250/255; 631/92/469; 631/92/552; Antibodies; Antigens; Biochemical Engineering; Biochemistry; Bioorganic Chemistry; Cell Biology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; DNA biosynthesis; G protein-coupled receptors; Glycoproteins; Immunization; Nanobodies; Severe acute respiratory syndrome coronavirus 2; Somatic hypermutation; Yeast; Yeasts

The predominant approach for antibody generation remains animal immunization, which can yield exceptionally selective and potent antibody clones owing to the powerful evolutionary process of somatic hypermutation. However, animal immunization is inherently slow, not always accessible and poorly comp...