Effects of fixing and staining on images of tropomyosin Mg-paracrystals

• Published in: Journal of microscopy (Oxford) Vol. 134; no. Pt 2; p. 137
• Main Authors: Lamvik, M K, White, L R, Eik-Nes, K B
• Format: Journal Article
• Language: English
• Published: England 01.05.1984
• Subjects: Animals; Crystallization; Histological Techniques; Hydrogen-Ion Concentration; Indicators and Reagents; Microscopy, Electron; Muscles; Rabbits; Staining and Labeling; Tropomyosin - analysis

Tropomyosin Mg-paracrystals have been studied by positive and negative staining with uranyl acetate and sodium phosphotungstate, either unfixed or fixed with glutaraldehyde. Fixation causes changes in some of the paracrystal bands. Some of the bands are more intense when a positively-charged stainin...