Theoretical Study on the Selective Fluorescence of PicoGreen: Binding Models and Photophysical Properties

• Published in: Bulletin of the Chemical Society of Japan Vol. 87; no. 2; pp. 267 - 273
• Main Authors: Okoshi, Masaki, Saparpakorn, Patchreenart, Takada, Yuta, Hannongbua, Supa, Nakai, Hiromi
• Format: Journal Article
• Language: English
• Published: The Chemical Society of Japan 15.02.2014
• Subjects: Binding; Deactivation; Deoxyribonucleic acid; Docking; Fluorescence; Grounds; Simulation; Stacking

PicoGreen (PG) is used as a probe to selectively quantitate double-stranded (ds-) DNA because it shows unique fluorescence enhancement when complexed with DNA. By binding to ds- and single-stranded (ss-) DNA, the quantum yields of PG–DNA complexes become remarkably larger than that of a free molecul...