ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D

• Published in: Nature (London) Vol. 630; no. 8016; pp. 437 - 446
• Main Authors: Du, Gang, Healy, Liam B., David, Liron, Walker, Caitlin, El-Baba, Tarick J., Lutomski, Corinne A., Goh, Byoungsook, Gu, Bowen, Pi, Xiong, Devant, Pascal, Fontana, Pietro, Dong, Ying, Ma, Xiyu, Miao, Rui, Balasubramanian, Arumugam, Puthenveetil, Robbins, Banerjee, Anirban, Luo, Hongbo R., Kagan, Jonathan C., Oh, Sungwhan F., Robinson, Carol V., Lieberman, Judy, Wu, Hao
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 13.06.2024; Nature Publishing Group
• Subjects: 101/28; 101/58; 13/109; 14/1; 14/19; 14/28; 631/250/262; 631/80/458; 82/80; 82/83; Acyltransferases - metabolism; Animals; Cell death; Cleavage; Cryoelectron Microscopy; Cysteine - metabolism; Electron microscopy; Female; Gasdermins - chemistry; Gasdermins - metabolism; Humanities and Social Sciences; Humans; Inflammasomes; Inflammasomes - metabolism; Inflammation; Liposomes - chemistry; Liposomes - metabolism; Lipoylation; Localization; Male; Mice; Microscopy; Mitochondria - metabolism; multidisciplinary; Palmitoylation; Phosphate-Binding Proteins - chemistry; Phosphate-Binding Proteins - metabolism; Polyethylene glycol; Pore formation; Pores; Pyroptosis; Reactive oxygen species; Reactive Oxygen Species - metabolism; Science; Science (multidisciplinary); THP-1 Cells

Gasdermin D (GSDMD) is the common effector for cytokine secretion and pyroptosis downstream of inflammasome activation and was previously shown to form large transmembrane pores after cleavage by inflammatory caspases to generate the GSDMD N-terminal domain (GSDMD-NT) 1 – 10 . Here we report that GS...