The NH2-Terminal Peptide of α-Smooth Muscle Actin Inhibits Force Generation by the Myofibroblast in vitro and in vivo

Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of α-smooth muscle actin (α-SMA), the actin isoform typical of...

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Published inThe Journal of cell biology Vol. 157; no. 4; pp. 657 - 663
Main Authors Hinz, Boris, Gabbiani, Giulio, Chaponnier, Christine
Format Journal Article
LanguageEnglish
Published United States Rockefeller University Press 13.05.2002
The Rockefeller University Press
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Abstract Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of α-smooth muscle actin (α-SMA), the actin isoform typical of vascular smooth muscle cells that has been suggested to play an important role in myofibroblast force generation. Actin isoforms differ slightly in their NH2-terminal sequences; these conserved differences suggest different functions. When the NH2-terminal sequence of α-SMA Ac-EEED is delivered to cultured myofibroblast in the form of a fusion peptide (FP) with a cell penetrating sequence, it inhibits their contractile activity; moreover, upon topical administration in vivo it inhibits the contraction of rat wound granulation tissue. The NH2-terminal peptide of α-skeletal actin has no effect on myofibroblasts, whereas the NH2-terminal peptide of β-cytoplasmic actin abolishes the immunofluorescence staining for this isoform without influencing α-SMA distribution and cell contraction. The FPs represent a new tool to better understand the specific functions of actin isoforms. Our findings support the crucial role of α-SMA in wound contraction. The α-SMA-FP will be useful for the understanding of the mechanisms of connective tissue remodeling; moreover, it furnishes the basis for a cytoskeleton-dependent preventive and/or therapeutic strategy for fibrocontractive pathological situations.
AbstractList Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of alpha-smooth muscle actin (alpha-SMA), the actin isoform typical of vascular smooth muscle cells that has been suggested to play an important role in myofibroblast force generation. Actin isoforms differ slightly in their NH2-terminal sequences; these conserved differences suggest different functions. When the NH2-terminal sequence of alpha-SMA Ac-EEED is delivered to cultured myofibroblast in the form of a fusion peptide (FP) with a cell penetrating sequence, it inhibits their contractile activity; moreover, upon topical administration in vivo it inhibits the contraction of rat wound granulation tissue. The NH2-terminal peptide of alpha-skeletal actin has no effect on myofibroblasts, whereas the NH2-terminal peptide of beta-cytoplasmic actin abolishes the immunofluorescence staining for this isoform without influencing alpha-SMA distribution and cell contraction. The FPs represent a new tool to better understand the specific functions of actin isoforms. Our findings support the crucial role of alpha-SMA in wound contraction. The alpha-SMA-FP will be useful for the understanding of the mechanisms of connective tissue remodeling; moreover, it furnishes the basis for a cytoskeleton-dependent preventive and/or therapeutic strategy for fibrocontractive pathological situations.
Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of alpha-smooth muscle actin, the actin isoform typical of vascular smooth muscle cells that has been suggested to play an important role in myofibroblast force generation.
Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of α–smooth muscle actin (α-SMA), the actin isoform typical of vascular smooth muscle cells that has been suggested to play an important role in myofibroblast force generation. Actin isoforms differ slightly in their NH 2 -terminal sequences; these conserved differences suggest different functions. When the NH 2 -terminal sequence of α-SMA Ac-EEED is delivered to cultured myofibroblast in the form of a fusion peptide (FP) with a cell penetrating sequence, it inhibits their contractile activity; moreover, upon topical administration in vivo it inhibits the contraction of rat wound granulation tissue. The NH 2 -terminal peptide of α–skeletal actin has no effect on myofibroblasts, whereas the NH 2 -terminal peptide of β–cytoplasmic actin abolishes the immunofluorescence staining for this isoform without influencing α-SMA distribution and cell contraction. The FPs represent a new tool to better understand the specific functions of actin isoforms. Our findings support the crucial role of α-SMA in wound contraction. The α-SMA–FP will be useful for the understanding of the mechanisms of connective tissue remodeling; moreover, it furnishes the basis for a cytoskeleton-dependent preventive and/or therapeutic strategy for fibrocontractive pathological situations.
Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive diseases. The marker of fibroblast-myofibroblast modulation is the neo expression of α-smooth muscle actin (α-SMA), the actin isoform typical of vascular smooth muscle cells that has been suggested to play an important role in myofibroblast force generation. Actin isoforms differ slightly in their NH2-terminal sequences; these conserved differences suggest different functions. When the NH2-terminal sequence of α-SMA Ac-EEED is delivered to cultured myofibroblast in the form of a fusion peptide (FP) with a cell penetrating sequence, it inhibits their contractile activity; moreover, upon topical administration in vivo it inhibits the contraction of rat wound granulation tissue. The NH2-terminal peptide of α-skeletal actin has no effect on myofibroblasts, whereas the NH2-terminal peptide of β-cytoplasmic actin abolishes the immunofluorescence staining for this isoform without influencing α-SMA distribution and cell contraction. The FPs represent a new tool to better understand the specific functions of actin isoforms. Our findings support the crucial role of α-SMA in wound contraction. The α-SMA-FP will be useful for the understanding of the mechanisms of connective tissue remodeling; moreover, it furnishes the basis for a cytoskeleton-dependent preventive and/or therapeutic strategy for fibrocontractive pathological situations.
Author Chaponnier, Christine
Gabbiani, Giulio
Hinz, Boris
AuthorAffiliation Department of Pathology, Centre Médical Universitaire, University of Geneva, 1211 Geneva 4, Switzerland
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Address correspondence to Giulio Gabbiani, Dept. of Pathology, CMU, University of Geneva, 1 rue Michel-Servet, 1211 Geneva 4, Switzerland. Tel.: 41-22-702-5742. Fax: 41-22-702-5746. E-mail: Giulio.Gabbiani@medecine.unige.ch
The online version of this article contains supplemental material.
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Publisher Rockefeller University Press
The Rockefeller University Press
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Snippet Myofibroblasts are specialized fibroblasts responsible for granulation tissue contraction and the soft tissue retractions occurring during fibrocontractive...
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jstor
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StartPage 657
SubjectTerms Actins
Actins - genetics
Actins - metabolism
Actins - pharmacology
Animals
Cell Movement - drug effects
Cell Movement - physiology
Cell Size - drug effects
Cell Size - physiology
Cells
Cells, Cultured
Cellular biology
Collagen Type I - biosynthesis
Collagen Type I - drug effects
Collagens
Cultured cells
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Female
Fibroblasts
Fibroblasts - cytology
Fibroblasts - drug effects
Fibroblasts - metabolism
Fluorescent Antibody Technique
Granulation tissue
Granulation Tissue - cytology
Granulation Tissue - drug effects
Granulation Tissue - metabolism
Messenger RNA
Muscle, Smooth - metabolism
Muscular system
Myofibroblasts
Peptides
Peptides - metabolism
Protein isoforms
Protein Structure, Tertiary
Rats
Rats, Wistar
Recombinant Fusion Proteins - pharmacology
RNA, Messenger - drug effects
RNA, Messenger - metabolism
Stress fibers
Stress, Mechanical
Tensile Strength - physiology
Washing
Wound Healing - drug effects
Wound Healing - physiology
Title The NH2-Terminal Peptide of α-Smooth Muscle Actin Inhibits Force Generation by the Myofibroblast in vitro and in vivo
URI https://www.jstor.org/stable/1621087
https://www.ncbi.nlm.nih.gov/pubmed/11994316
https://www.proquest.com/docview/217092072
https://search.proquest.com/docview/71688277
https://pubmed.ncbi.nlm.nih.gov/PMC2173846
Volume 157
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