Conventional Freezing, Straw, and Open-Pulled Straw Vitrification of Mouse Two Pronuclear (2-PN) Stage Embryos

• Published in: Animal biotechnology Vol. 18; no. 3; pp. 203 - 212
• Main Authors: Zhao, Xue-Ming, Quan, Guo-Bo, Zhou, Guang-Bin, Hou, Yun-Peng, Zhu, Shi-En
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Group 01.01.2007
• Subjects: Animals; Conventional freezing; Cryopreservation - veterinary; Cryoprotective Agents; Dimethyl Sulfoxide; Embryo Transfer - veterinary; Ethylene Glycol; Female; Ficoll; Male; Mice - embryology; Morula; Mouse; Open-pulled straw (OPS); Pregnancy; Sucrose; Two-pronuclear embryo; Vitrification

Little is known on the cryopreservation of mouse pronuclear (PN) stage embryos. In the present experiment the mouse 2-PN stage embryos were cryopreserved by conventional freezing, straw, or open-pulled straw (OPS) vitrificaiton methods. The conventional freezing solution was 1.5 mol/L ethylene glyco...