Structural characterization and immunomodulatory activity of a new polysaccharide from jellyfish

• Published in: Carbohydrate polymers Vol. 159; pp. 188 - 194
• Main Authors: Li, Qiang-Ming, Wang, Jing-Fei, Zha, Xue-Qiang, Pan, Li-Hua, Zhang, Hai-Lin, Luo, Jian-Ping
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.03.2017
• Subjects: Immunomodulatory activity; Jellyfish; Polysaccharide; Structural characterization; Jellyfish; Immunomodulatory activity; Polysaccharide; Structural characterization
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2016.12.031

•A homogenous polysaccharide (JSP-11) was isolated from jellyfish.•The structural features of JSP-11 were characterized.•The activation of macrophages by JSP-11 and the related mechanism were studied. A new polysaccharide (JSP-11) with a molecular weight of 1.25×106Da was extracted and purified from jellyfish. Monosaccharide analysis showed that JSP-11 was composed of mannose, galactose and glucuronic acid with a molar ratio of 2.18:1.00:1.94. According to the analysis of fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, JSP-11 was determined to contain a linear backbone which consisted of (1→3,6)-linked β-d-Manp and (1→6)-linked β-d-Galp. The branch of (1→)-linked α-d-GlcpA was attached to the C-3 position of (1→3,6)-linked β-d-Manp in the backbone. The immunomodulatory assay exhibited that JSP-11 could significantly enhance the viability of RAW 264.7 macrophage cells, and promote the release of NO, TNF-α, and IL-1β via activating NF-κB, MAPKs and PI3K/Akt signal pathways.