Use of ELISA to detect toxigenic Pasteurella multocida in atrophic rhinitis in swine

• Published in: Journal of veterinary diagnostic investigation Vol. 4; no. 4; p. 419
• Main Authors: Bowersock, T.L. (Purdue University, W. Lafayette, IN), Hooper, T, Pottenger, R
• Format: Journal Article
• Language: English
• Published: United States 01.10.1992
• Subjects: Animals; ATROPHIC RHINITIS; Bacterial Toxins - biosynthesis; CERDO; CULTIVO DE TEJIDOS; CULTURE DE TISSU; Dermotoxins - biosynthesis; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; ELISA; Enzyme-Linked Immunosorbent Assay - veterinary; INDIANA; Nasal Mucosa - microbiology; Pasteurella Infections - veterinary; PASTEURELLA MULTOCIDA; Pasteurella multocida - isolation & purification; Pasteurella multocida - metabolism; PIGS; PORCIN; Prevalence; RHINITE ATROPHIQUE; Rhinitis, Atrophic - microbiology; Rhinitis, Atrophic - veterinary; RINITIS ATROFICA; Sensitivity and Specificity; SWINE; Swine Diseases - microbiology; TEST ELISA; TISSUE CULTURE
• ISSN: 1040-6387; 1943-4936
• DOI: 10.1177/104063879200400409

The use of an enzyme-linked immunosorbent assay (ELISA) as a means of detecting dermonecrotoxin-producing strains of Pasteurella multocida was investigated. The assay was evaluated as a means to identify toxigenic P. multocida isolates recovered from nasal secretions of swine with atrophic rhinitis. The sensitivity and specificity of the ELISA for detecting dermonecrotoxin-producing P. multocida strains were compared to those of mouse-inoculation and cytotoxicity assays. The ELISA was highly sensitive and more specific than animal inoculation or tissue culture assay and is thus a more effective method for screening swine herds for the presence of toxigenic strains of P. multocida. The ELISA is a rapid, effective, economical way to identify toxigenic P. multocida isolates.