Comparison of Echinostoma caproni mother sporocyst development in vivo and in vitro using Biomphalaria glabrata snails and a B. glabrata embryonic cell line
Biomphalaria glabrata embryonic (Bge) cells have previously been shown to permit a successful cocultivation of Schistosoma mansoni and Schistosoma japonicum from miracidia to mother sporocysts (MS) and then to the production of daughter sporocysts (DS). To investigate further the properties of the B...
Saved in:
Published in | The Journal of parasitology Vol. 84; no. 2; p. 227 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
United States
01.04.1998
|
Subjects | |
Online Access | Get more information |
Cover
Loading…
Abstract | Biomphalaria glabrata embryonic (Bge) cells have previously been shown to permit a successful cocultivation of Schistosoma mansoni and Schistosoma japonicum from miracidia to mother sporocysts (MS) and then to the production of daughter sporocysts (DS). To investigate further the properties of the Bge culturing system we used Echinostoma caproni under identical in vitro conditions. In vitro-derived miracidia were used either for experimental infections of B. glabrata snails, or for in vitro cultivation with Bge cells. Histological analysis showed that the development of MS in B. glabrata was similar to the previously described development in Biomphalaria pfeifferi in terms of final site of infection, development dynamics, growth dynamics, reproduction intensity, and life spans. Only short delays in migration dynamics were observed in B. glabrata. When cultivated under in vitro conditions, E. caproni MS could live for up to 17 wk in the presence of Bge cells, as compared with 2 wk in cell-free Bge medium. The presence of Bge cells also permitted significant growth of MS and development through complete embryogenesis of the next intramolluscan stage (embryos of 100-110 cells). However, degeneration of MS consistently occurred before production of this second generation. During the entire cultivation period, no visible contact was observed between MS and Bge cells, suggesting that development of MS was only triggered by soluble factors released by Bge cells. |
---|---|
AbstractList | Biomphalaria glabrata embryonic (Bge) cells have previously been shown to permit a successful cocultivation of Schistosoma mansoni and Schistosoma japonicum from miracidia to mother sporocysts (MS) and then to the production of daughter sporocysts (DS). To investigate further the properties of the Bge culturing system we used Echinostoma caproni under identical in vitro conditions. In vitro-derived miracidia were used either for experimental infections of B. glabrata snails, or for in vitro cultivation with Bge cells. Histological analysis showed that the development of MS in B. glabrata was similar to the previously described development in Biomphalaria pfeifferi in terms of final site of infection, development dynamics, growth dynamics, reproduction intensity, and life spans. Only short delays in migration dynamics were observed in B. glabrata. When cultivated under in vitro conditions, E. caproni MS could live for up to 17 wk in the presence of Bge cells, as compared with 2 wk in cell-free Bge medium. The presence of Bge cells also permitted significant growth of MS and development through complete embryogenesis of the next intramolluscan stage (embryos of 100-110 cells). However, degeneration of MS consistently occurred before production of this second generation. During the entire cultivation period, no visible contact was observed between MS and Bge cells, suggesting that development of MS was only triggered by soluble factors released by Bge cells. |
Author | Fournier, A Coustau, C Ataev, G L |
Author_xml | – sequence: 1 givenname: G L surname: Ataev fullname: Ataev, G L organization: Department of Invertebrate Zoology, Saint-Petersburg University, Russia – sequence: 2 givenname: A surname: Fournier fullname: Fournier, A – sequence: 3 givenname: C surname: Coustau fullname: Coustau, C |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/9576492$$D View this record in MEDLINE/PubMed |
BookMark | eNpFkM1qAjEUhbOwWLWlT1DIC4yNSWbGWVaxPyB0067lJrnRlEwyJKPgu_RhO1Shi8O5cA7fhTMloxADEvKwYHMuWP0k-FLKuhyRCWOcF0I05S2Z5vzNGCsHjcm4KetKNnxCftax7SC5HAONlm70wYWY-9gC1dClGBxtY3_ARHMXU9Tn3FODJ_SxazH01AV6cqdIIZjL3adIj9mFPV25AX0AP9CB7j2oBD3QHMD5_NcHupr_B9iqdB7-aarRe-pdwDtyY8FnvL_6jHy9bD7Xb8X24_V9_bwttBSLvpCl4BYqqHnVKMG5sBKFRc2NYdpKbrhVZYmgUCwFVloyqY1CAxWzS6WBz8jjhdsdVYtm1yXXQjrvrivxX2hsbF4 |
CitedBy_id | crossref_primary_10_1186_s13071_018_3059_2 crossref_primary_10_1017_S0031182009991302 crossref_primary_10_1645_19_36 crossref_primary_10_1006_expr_1999_4462 crossref_primary_10_1016_j_dci_2019_103557 crossref_primary_10_1016_j_jip_2011_12_006 crossref_primary_10_1645_0022_3395_2001_087_1160_GEATDI_2_0_CO_2 crossref_primary_10_3389_fmars_2022_936520 crossref_primary_10_1016_S0145_305X_99_00023_3 crossref_primary_10_1016_j_actatropica_2022_106636 crossref_primary_10_1016_j_exppara_2004_11_005 crossref_primary_10_1007_s00436_018_5930_7 |
ContentType | Journal Article |
DBID | CGR CUY CVF ECM EIF NPM |
DOI | 10.2307/3284475 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
DatabaseTitleList | MEDLINE |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | no_fulltext_linktorsrc |
Discipline | Zoology Biology |
ExternalDocumentID | 9576492 |
Genre | Research Support, Non-U.S. Gov't Journal Article Comparative Study |
GroupedDBID | --- -JH -~X .55 .GJ 0R~ 123 29L 2AX 36B 3O- 3V. 4R4 53G 5GY 5RE 692 79B 7X7 88A 88E 8AO 8C1 8CJ 8FE 8FH 8FI 8FJ AAHBH AAHKG AAPSS ABBHK ABOCM ABPLY ABPPZ ABTAH ABTLG ABUWG ABXSQ ACGFO ACIHN ACNCT ACPRK ADACV ADBBV ADHSS ADULT AEAQA AENEX AEPYG AEUPB AFAZZ AFFIJ AFFNX AFKRA AFNWH AFRAH AGUYK AHMBA AI. AKPMI ALIPV ALMA_UNASSIGNED_HOLDINGS AQVQM AS~ BBNVY BENPR BHPHI BPHCQ BVXVI CAG CBGCD CCPQU CGR COF CS3 CUY CVF D1J DC7 DOOOF DU5 EBD EBS ECGQY ECM EIF EJD EMB EMOBN F5P FRJ FYUFA G8K GTFYD H13 HCIFZ HGD HMCUK HTVGU HZ~ IAO IEA IFM IGS IL9 INH INR IPSME ISR JAAYA JBMMH JBS JENOY JHFFW JKQEH JLS JLXEF JPM JSODD JST KQ8 L7B LK8 LU7 M0L M1P M7P MV1 NPM NVHAQ O9- OK1 OVD P2P PQ0 PQQKQ PROAC PSQYO Q5J QF4 QM4 QN7 QO4 RBO ROL SA0 SJN SV3 TEORI TR2 UKHRP UPT VH1 VQA WH7 WHG X7M XOL Y3D YQT ZCA ZCG ZGI ZY4 ~02 ~EF ~KM |
ID | FETCH-LOGICAL-c431t-4532fa6a7269b3223f4e3fec2dd0cf42d2fb55eabe383e6c404cdbeda60f8bca2 |
ISSN | 0022-3395 |
IngestDate | Sat Sep 28 07:42:08 EDT 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 2 |
Language | English |
LinkModel | OpenURL |
MergedId | FETCHMERGED-LOGICAL-c431t-4532fa6a7269b3223f4e3fec2dd0cf42d2fb55eabe383e6c404cdbeda60f8bca2 |
PMID | 9576492 |
ParticipantIDs | pubmed_primary_9576492 |
PublicationCentury | 1900 |
PublicationDate | 1998-04-01 |
PublicationDateYYYYMMDD | 1998-04-01 |
PublicationDate_xml | – month: 04 year: 1998 text: 1998-04-01 day: 01 |
PublicationDecade | 1990 |
PublicationPlace | United States |
PublicationPlace_xml | – name: United States |
PublicationTitle | The Journal of parasitology |
PublicationTitleAlternate | J Parasitol |
PublicationYear | 1998 |
SSID | ssj0005000 |
Score | 1.6598564 |
Snippet | Biomphalaria glabrata embryonic (Bge) cells have previously been shown to permit a successful cocultivation of Schistosoma mansoni and Schistosoma japonicum... |
SourceID | pubmed |
SourceType | Index Database |
StartPage | 227 |
SubjectTerms | Animals Biomphalaria - cytology Biomphalaria - embryology Biomphalaria - parasitology Cell Line Echinostoma - growth & development Echinostoma - ultrastructure Microscopy, Electron, Scanning |
Title | Comparison of Echinostoma caproni mother sporocyst development in vivo and in vitro using Biomphalaria glabrata snails and a B. glabrata embryonic cell line |
URI | https://www.ncbi.nlm.nih.gov/pubmed/9576492 |
Volume | 84 |
hasFullText | |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Ja9wwFBaTlkAupWkbugYdehOeOpZsj4-ZkBJKyCmB0EuQZIm6ZOwh4wlMf0eP_bF9WmwpQ1vaXoywbGHrfbxNb0HovU5zrkHzTdKyLBOW1SoBIVwlVGsQgFVp6o2YaIuL4uyKfbrOryeT71HU0roXU_ntl3kl_0NVuAd0NVmy_0DZcVG4AWOgL1yBwnD9KxqfxE0EyakJizR1MhacSL40RW_JwiZYEWO7dnKz6ockKRsC0LTkvrnvfAEmGPd3HVlb58G8gaW_cDB7G05Awwabuudk1fLm1hV15mQ-DRNqIe42tpeOOQggt8Nh_deAxUjzNeXGV03_wKF_3HP4e-OkJ-dBMsIrjW-3PR6WdCblax0cvHXI4RsjXaIUAuq6aw5s2HWK83DLYp7qigds83ofwU5BvjLXfCWi-HJhSV6BNcVcr70_Tm6V3PYzO2innBmueWE8QEPYUOrTmvwvhETsD_5T9tCuX2DLVLEqy-VT9MTvOD52wNlHE9U-Q7uu--gGRp87O3qOfgQg4U7jCEjYAwk7IOERSDgCEm5abICEARhuDEDCFkg4BhIe8IIdkOzzHM-nYWIEEjZAwgZIL9DVx9PLk7PEt-1IJGijfcJymmle8DIrKgHygmqmqFYyq-tUamAHmRZ5rrhQdEZVIVnKZC1UzYtUz4Tk2QF61HateokwyJ9iRnlOU6VYXheVAu4h5dERz2qQNuIVOnAbfLN0tVlu_M6__t3EG7QXEPkWPdbACtQ70Ct7cWgJ_RMOKX5I |
link.rule.ids | 786 |
linkProvider | National Library of Medicine |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Comparison+of+Echinostoma+caproni+mother+sporocyst+development+in+vivo+and+in+vitro+using+Biomphalaria+glabrata+snails+and+a+B.+glabrata+embryonic+cell+line&rft.jtitle=The+Journal+of+parasitology&rft.au=Ataev%2C+G+L&rft.au=Fournier%2C+A&rft.au=Coustau%2C+C&rft.date=1998-04-01&rft.issn=0022-3395&rft.volume=84&rft.issue=2&rft.spage=227&rft_id=info:doi/10.2307%2F3284475&rft_id=info%3Apmid%2F9576492&rft_id=info%3Apmid%2F9576492&rft.externalDocID=9576492 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0022-3395&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0022-3395&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0022-3395&client=summon |