Reduced bacterial adhesion on zirconium-based bulk metallic glasses by femtosecond laser nanostructuring

• Published in: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of engineering in medicine Vol. 234; no. 4; p. 387
• Main Authors: Du, Cezhi, Wang, Chengyong, Zhang, Tao, Yi, Xin, Liang, Jianyi, Wang, Hongjian
• Format: Journal Article
• Language: English
• Published: England 01.04.2020
• Subjects: 3T3 Cells; Animals; Bacterial Adhesion - drug effects; Glass - chemistry; Lasers; Mice; Nanostructures; Surface Properties; Zirconium - chemistry; Zirconium - pharmacology; nanostructure; Bacterial adhesion; bulk metallic glass; femtosecond laser
• DOI: 10.1177/0954411919898011

As high-performing materials, bulk metallic glasses have attracted widespread attention for biomedical applications. Herein, the bacterial adhesion properties of femtosecond laser-nanostructured surfaces of four types of zirconium-based bulk metallic glasses are assessed. Laser-induced periodical surface structures and nanoparticle structures were fabricated by femtosecond laser irradiation under different energy intensities (0.23 and 2.3 J/mm ). Surface topography, roughness, wettability, and surface energy were investigated after femtosecond laser irradiation and the surface bacterial adhesion properties were explored using and as respective representatives of Gram-negative and Gram-positive bacteria. 4',6-Diamidino-2-phenylindole fluorescence staining was used to characterize and assess the bacterial surface coverage rate. The cytotoxicity of polished and laser-nanostructured surfaces was investigated using MC3T3-E cells. The obtained results demonstrate that femtosecond laser surface nanostructuring retained the amorphous structure of zirconium-based bulk metallic glasses and led to an obvious decrease in bacterial adhesion compared with polished surfaces. The inhibition of bacterial adhesion on laser-induced periodical surface structures was greater than on nanostructured surfaces after 24 h of bacterial incubation. In addition, femtosecond laser nanostructuring did not have an apparent effect on the cytotoxicity of zirconium-based bulk metallic glasses.