Standardization and validation of a novel UPLC-MS/MS method to quantify first line anti-tuberculosis drugs in plasma and dried blood spots

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1228; p. 123801
• Main Authors: Herrera-Pérez, Ivette Guadalupe, Rodríguez-Báez, Ana Socorro, Ortiz-Álvarez, Arturo, Velarde-Salcedo, Rodrigo, Arriaga-García, Francisco Javier, Rodríguez-Pinal, Cristian Jazmín, Romano-Moreno, Silvia, Milán-Segovia, Rosa del Carmen, Medellín-Garibay, Susanna Edith
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2023
• Subjects: Dried blood spots; First line anti-tuberculosis drugs; Therapeutic drug monitoring; Tuberculosis; UPLC-MS/MS; LLOQ; INH; MRM; Cp; Therapeutic drug monitoring; CCs; DBS; ME; ESI; EMB; TDM; QCs; LOD; Cmax; Dried blood spots; First line anti-tuberculosis drugs; DOTS; TB; Tuberculosis; CV; RMP; UPLS-MS/MS; PZA; Tmax; Hct; UPLC-MS/MS

•UPLC-MS/MS conditions were standardized to quantify first-line antiTB drugs.•Bioanalytical methods based on microsampling strategies were validated.•AntiTB drugs were quantified in plasma and capillary blood from TB patients.•Clinical applicability of bioanalytical methods was demonstrated for TDM...