A sensitive LC–MS/MS assay for the simultaneous analysis of the major active components of silymarin in human plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 902; pp. 1 - 9
• Main Authors: Brinda, Bryan J., Zhu, Hao-Jie, Markowitz, John S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2012
• Subjects: ammonium acetate; Assaying; Chromatography; Chromatography, Liquid - methods; Constituents; Drug Stability; Extraction plants; Female; formic acid; fruits; Human; Humans; LC–MS/MS; Liquid-Liquid Extraction; Liquids; Male; mass spectrometry; methanol; Methyl alcohol; Milk; Milk thistle; Plant Extracts; Reproducibility of Results; Sensitivity and Specificity; Silybin; Silybum marianum; Silymarin; Silymarin - blood; Silymarin - chemistry; Silymarin - pharmacokinetics; Tandem Mass Spectrometry - methods; Taxifolin; Silybin; Milk thistle; Taxifolin; Silymarin; LC–MS/MS

► Silymarin extract contains numerous bioactive flavonolignans and flavonoids. ► Analysis of silychristin, silydianin, silybin A and B, isosilybin A and B, and taxifolin. ► Pure standard compounds were utilized for absolute quantification. ► The lower limit of quantification was 2ng/ml for each anal...