Celebrating 50 years of fluorescence correlation spectroscopy (FCS): Advancing live-cell massively parallel FCS studies with photostable GFPs, mStayGold and StayGold/E138D

• Published in: Biochimica et biophysica acta. General subjects Vol. 1869; no. 7; p. 130809
• Main Authors: Oasa, Sho, Stoyanov, Borislav, Hamada, Yuta, Nikolić, Stanko N., Krmpot, Aleksandar J., Kitamura, Akira, Vukojević, Vladana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2025
• Subjects: Active Transport, Cell Nucleus; Apparent brightness analysis; exports; fluorescence correlation spectroscopy; Fluorescence correlation spectroscopy (FCS) and massively parallel FCS; Glucocorticoid receptor; glucocorticoid receptors; green fluorescent protein; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; HEK293 Cells; Homodimerization; Humans; mStayGold; nuclear membrane; Nucleocytoplasmic transport; photostability; Protein Multimerization; Receptors, Glucocorticoid - chemistry; Receptors, Glucocorticoid - metabolism; signal-to-noise ratio; Spectrometry, Fluorescence - methods; Nucleocytoplasmic transport; Fluorescence correlation spectroscopy (FCS) and massively parallel FCS; Apparent brightness analysis; mStayGold; Glucocorticoid receptor; Homodimerization

More than 50 years after its inception, fluorescence correlation spectroscopy (FCS) remains a cornerstone technique for quantitative characterization of the cellular dynamics of molecules and their concentration and interactions in live cells. The enhanced green fluorescent protein (eGFP) has long b...