Long-term monitoring in a microfluidic system to study tumour spheroid response to chronic and cycling hypoxia

• Published in: Scientific reports Vol. 9; no. 1; pp. 17782 - 13
• Main Authors: Grist, Samantha M, Nasseri, S Soroush, Laplatine, Loïc, Schmok, Jonathan C, Yao, Dickson, Hua, Jessica, Chrostowski, Lukas, Cheung, Karen C
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 28.11.2019; Nature Publishing Group UK
• Subjects: Antibiotics, Antineoplastic - pharmacokinetics; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cell culture; Doxorubicin; Doxorubicin - pharmacokinetics; Equipment Design; Female; Humans; Hypoxia; MCF-7 Cells; Microfluidic Analytical Techniques - instrumentation; Microfluidics; Microscopy; Neoplasms - drug therapy; Neoplasms - metabolism; Neoplasms - pathology; Oxygen; Oxygen - metabolism; Sectioning; Spheroids; Spheroids, Cellular; Tumor Cells, Cultured; Tumor Hypoxia - drug effects; Tumors

We demonstrate the application of a microfluidic platform combining spatiotemporal oxygen control and long-term microscopy monitoring to observe tumour spheroid response to hypoxia. The platform is capable of recreating physiologically-relevant low and cycling oxygen levels not attainable in traditi...