Animal model of assessing cerebrovascular functional reserve by imaging photoplethysmography

• Published in: Scientific reports Vol. 10; no. 1; p. 19008
• Main Authors: Mamontov, Oleg V, Sokolov, Alexey Y, Volynsky, Maxim A, Osipchuk, Anastasija V, Zaytsev, Valery V, Romashko, Roman V, Kamshilin, Alexei A
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 04.11.2020; Nature Publishing Group UK
• Subjects: Animal models; Animals; Blood flow; Blood pressure; Carbon dioxide; Carbonic Anhydrase Inhibitors - administration & dosage; Carbonic anhydrases; Cerebral blood flow; Cerebrovascular Circulation - drug effects; Cerebrovascular diseases; Cerebrovascular Disorders - physiopathology; EKG; Feasibility studies; Heart Rate - drug effects; Injection; Male; Models, Animal; Neuroimaging; Neurosurgery; Photoplethysmography - methods; Rats, Wistar; Sulfonamides - administration & dosage; Thiophenes - administration & dosage; Vascular diseases
• DOI: 10.1038/s41598-020-75824-w
• ISSN: 2045-2322

Assessment of the cerebral blood-flow-reserve in patients with cerebrovascular diseases is extremely important in terms of making prognosis, determining treatment tactics, and controlling the revascularization outcome in the case of reconstructive interventions on the brain vessels. However, there is no easy-to-use, contactless method for either assessing the functional reserve of the cortical vascular network or intraoperative monitoring of surgical intervention. Our study aims to demonstrate feasibility of green-light imaging photoplethysmography (iPPG) to estimate cerebrovascular functional reserve in animal model of craniosurgical intervention. Custom-made iPPG system was exploited to visualize intracranial vessels in anesthetized Wistar rats (n = 15). Video frames of rat's cortex were recorded concurrently with systemic blood pressure, end-tidal CO , and electrocardiogram. We found that injection of dorzolamide (carbonic-anhydrase inhibitor) significantly increased the blood-pulsations amplitude in all animals by 35 ± 19% (p < 0.001). Such an increase negatively correlated with significant decrease in end-tidal CO by 32 ± 7% (p < 0.001). It is noteworthy that the dorzolamide injection did not lead to significant changes in systemic blood pressure. Concluding, pulsations amplitude is a marker of the vascular tone that can be used to evaluate the functional cerebrovascular reserve. Imaging PPG is a simple and convenient method to assess cerebral blood flow, including during various neurosurgical interventions.