The use of high resolution melting (HRM) to map single nucleotide polymorphism markers linked to a covered smut resistance gene in barley

• Published in: Theoretical and applied genetics Vol. 117; no. 5; pp. 721 - 728
• Main Authors: Lehmensiek, Anke, Sutherland, Mark W., McNamara, Raymond B.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2008; Springer; Springer Nature B.V
• Subjects: Agriculture; Barley; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chromosome Mapping; Chromosome Mapping - methods; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; Expressed Sequence Tags; Fundamental and applied biological sciences. Psychology; Fungi; genes; Genes, Plant; Genetic Linkage; Genetic Markers; genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomes; genomics; haploidy; Hordeum; Hordeum - genetics; Hordeum - microbiology; Immunity, Innate; Immunity, Innate - genetics; Life Sciences; loci; melting; methods; Methods, theories and miscellaneous; microbiology; Original Paper; Phenotype; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Genetics and Genomics; Polymorphism; Polymorphism, Single Nucleotide; Pteridophyta, spermatophyta; rice; Seeds; single nucleotide polymorphism; Ustilago; Vegetals; Australia; Cleave Amplify Polymorphic Sequence; Standard Polymerase Chain Reaction; Single Nucleotide Polymorphism Marker; High Resolution Melting; Doubled Haploid; Genetic mapping; Resistance; Genetics; Agriculture; Single nucleotide polymorphism; Gene

Using an established genetic map, a single gene conditioning covered smut resistance, Ruh.7H , was mapped to the telomere region of chromosome 7HS in an Alexis/Sloop doubled haploid barley population. The closest marker to Ruh.7H , abg704 was 7.5 cM away. Thirteen loci on the distal end of 7HS with...