Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strains

• Published in: Nature microbiology Vol. 8; no. 3; pp. 410 - 423
• Main Authors: Apjok, Gábor, Számel, Mónika, Christodoulou, Chryso, Seregi, Viktória, Vásárhelyi, Bálint Márk, Stirling, Tamás, Eszenyi, Bálint, Sári, Tóbiás, Vidovics, Fanni, Nagrand, Erika, Kovács, Dorina, Szili, Petra, Lantos, Ildikó Ilona, Méhi, Orsolya, Jangir, Pramod K, Herczeg, Róbert, Gálik, Bence, Urbán, Péter, Gyenesei, Attila, Draskovits, Gábor, Nyerges, Ákos, Fekete, Gergely, Bodai, László, Zsindely, Nóra, Dénes, Béla, Yosef, Ido, Qimron, Udi, Papp, Balázs, Pál, Csaba, Kintses, Bálint
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 01.03.2023; Nature Publishing Group UK
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Antibiotics; Bacteria; Bacteria - genetics; Bacteriophages - genetics; Drug resistance; Genes, Bacterial; Genomes; Host specificity; Intestinal microflora; Metagenomics; Microbiomes; Phages; Site-directed mutagenesis; Species; Strains (organisms)

Functional metagenomics is a powerful experimental tool to identify antibiotic resistance genes (ARGs) in the environment, but the range of suitable host bacterial species is limited. This limitation affects both the scope of the identified ARGs and the interpretation of their clinical relevance. He...