Engineered virus-encoded pre-microRNA (pre-miRNA) induces sequence-specific antiviral response in addition to nonspecific immunity in a fish cell line: Convergence of RNAi-related pathways and IFN-related pathways in antiviral response

• Published in: Antiviral research Vol. 80; no. 3; pp. 316 - 323
• Main Authors: Dang, Lua T., Kondo, Hidehiro, Aoki, Takashi, Hirono, Ikuo
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.12.2008; Elsevier
• Subjects: Animals; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiviral agents; Antiviral Agents - immunology; Base Sequence; Biological and medical sciences; Cell Line; Fish Diseases - immunology; Fish Diseases - virology; Fish Proteins - genetics; Fish Proteins - immunology; Fishes; Genetic Engineering; Hirame rhabdovirus; HIRRV; IFN; Immunity, Innate; Interferon; Interferons - genetics; Interferons - immunology; Iridovirus; Iridovirus - genetics; Iridovirus - metabolism; MCP; Medical sciences; MicroRNAs - genetics; miRNA; Molecular Sequence Data; Mx protein; Novirhabdovirus - genetics; Novirhabdovirus - metabolism; Pharmacology. Drug treatments; RNA Interference; RNA, Small Interfering - genetics; RNAi; RSIV; Sequence Alignment; siRNA; IFN; RNAi; RSIV; miRNA; Interferon; siRNA; MCP; Mx protein; HIRRV; RNA interference; Cytokine; Micro RNA; Immunity; In vitro; Convergence; Virus; Gene silencing; Cell line; Antiviral

Transfection with synthesized virus-specific small interfering RNAs (siRNAs) efficiently inhibits viral replication in viral-infected fish cell lines, implying the involvement of RNA interference (RNAi)-related pathways in the antiviral response of fish cells. Here, we demonstrate that plasmid expre...