Single-Cell Transcriptomic Analysis of Human Lung Provides Insights into the Pathobiology of Pulmonary Fibrosis

• Published in: American journal of respiratory and critical care medicine Vol. 199; no. 12; pp. 1517 - 1536
• Main Authors: Reyfman, Paul A., Walter, James M., Joshi, Nikita, Anekalla, Kishore R., McQuattie-Pimentel, Alexandra C., Chiu, Stephen, Fernandez, Ramiro, Akbarpour, Mahzad, Chen, Ching-I, Ren, Ziyou, Verma, Rohan, Abdala-Valencia, Hiam, Nam, Kiwon, Chi, Monica, Han, SeungHye, Gonzalez-Gonzalez, Francisco J., Soberanes, Saul, Watanabe, Satoshi, Williams, Kinola J. N., Flozak, Annette S., Nicholson, Trevor T., Morgan, Vince K., Winter, Deborah R., Hinchcliff, Monique, Hrusch, Cara L., Guzy, Robert D., Bonham, Catherine A., Sperling, Anne I., Bag, Remzi, Hamanaka, Robert B., Mutlu, Gökhan M., Yeldandi, Anjana V., Marshall, Stacy A., Shilatifard, Ali, Amaral, Luis A. N., Perlman, Harris, Sznajder, Jacob I., Argento, A. Christine, Gillespie, Colin T., Dematte, Jane, Jain, Manu, Singer, Benjamin D., Ridge, Karen M., Lam, Anna P., Bharat, Ankit, Bhorade, Sangeeta M., Gottardi, Cara J., Budinger, G. R. Scott, Misharin, Alexander V.
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 15.06.2019
• Subjects: Animals; Biopsy; Cells, Cultured - pathology; Critical care; Disease; Disease Models, Animal; Epithelial Cells - pathology; Female; Flow cytometry; Gene expression; Histology; Humans; Idiopathic Pulmonary Fibrosis - genetics; Idiopathic Pulmonary Fibrosis - pathology; Male; Medicine; Original; Pathogenesis; Patients; Pulmonary fibrosis; Sequence Analysis, RNA; Stem Cells - pathology; Thoracic surgery; Transcriptome; Transplants & implants; Illinois; Chicago Illinois; RNA sequencing; alveolar type II cells; alveolar macrophages; pulmonary fibrosis

The contributions of diverse cell populations in the human lung to pulmonary fibrosis pathogenesis are poorly understood. Single-cell RNA sequencing can reveal changes within individual cell populations during pulmonary fibrosis that are important for disease pathogenesis. To determine whether singl...