FGF-1-Dependent Proliferative and Migratory Responses Are Impaired in Senescent Human Umbilical Vein Endothelial Cells and Correlate with the Inability to Signal Tyrosine Phosphorylation of Fibroblast Growth Factor Receptor-1 Substrates

• Published in: The Journal of cell biology Vol. 134; no. 3; pp. 783 - 791
• Main Authors: Garfinkel, S., Hu, X., Prudovsky, I. A., McMahon, G. A., Kapnik, E. M., McDowell, S. D., Maciag, T.
• Format: Journal Article
• Language: English
• Published: United States Rockefeller University Press 01.08.1996; The Rockefeller University Press
• Subjects: 3T3 cells; Antibodies; Base Sequence; Cell Adhesion; Cell Cycle; Cell Division; Cell growth; Cell Movement; Cells; Cells, Cultured; Cellular biology; Cellular Senescence; Cortactin; Endothelial cells; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Fetuses; Fibroblast Growth Factor 1 - pharmacology; Fibroblasts; Focal adhesions; Gene Expression Regulation - drug effects; Human umbilical vein endothelial cells; Humans; Microfilament Proteins - metabolism; Molecular Sequence Data; Phosphorylation; Proto-Oncogene Proteins pp60(c-src) - metabolism; Receptor Protein-Tyrosine Kinases; Receptor, Fibroblast Growth Factor, Type 1; Receptors, Fibroblast Growth Factor - metabolism; Receptors, Fibroblast Growth Factor - physiology; Signal Transduction - physiology; Tyrosine - metabolism; Umbilical Veins

Senescent cells do not proliferate in response to exogenous growth factors, yet the number and affinity of growth factor receptors on the cell surface appear to be similar to presenescent cell populations. To determine whether a defect in receptor signaling exists, we analyzed human umbilical vein e...