Effect of regulated expression of human cyclooxygenase isoforms on eicosanoid and isoeicosanoid production in inflammation

• Published in: The Journal of clinical investigation Vol. 105; no. 10; pp. 1473 - 1482
• Main Authors: McAdam, B.F., Mardini, I.A., Habib, A., Burke, A., Lawson, J.A., Kapoor, S., FitzGerald, G.A.
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 15.05.2000
• Subjects: Adult; Aspirin - pharmacology; Celecoxib; Cyclooxygenase 1; Cyclooxygenase 2; Cyclooxygenase 2 Inhibitors; Cyclooxygenase Inhibitors - pharmacology; Eicosanoids - biosynthesis; Female; Humans; Ibuprofen - pharmacology; In Vitro Techniques; Inflammation - etiology; Inflammation - metabolism; Isoenzymes - biosynthesis; Lipid Peroxidation - drug effects; Lipopolysaccharides - toxicity; Male; Membrane Proteins; Oxidative Stress - drug effects; Prostaglandin-Endoperoxide Synthases - biosynthesis; Prostaglandins - biosynthesis; Prostaglandins - urine; Pyrazoles; Sulfonamides - pharmacology

To examine the role of cyclooxygenase (COX) isozymes in prostaglandin formation and oxidant stress in inflammation, we administered to volunteer subjects placebo or bolus injections of lipopolysaccharide (LPS), which caused a dose-dependent increase in temperature, heart rate, and plasma cortisol. L...