Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes

The effect of dietary flavonoids (flavone, isoflavone, flavanone, chalcone, flavonol, and flavonol derivatives) on the differentiation of 3T3-L1 adipocytes was investigated. Glycerol-3-phosphate dehydrogenase (GPDH) activity, which is a hallmark of 3T3-L1 differentiation, was measured. The GPDH acti...

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Published inFood Science and Technology Research Vol. 7; no. 2; pp. 154 - 160
Main Authors IWASHITA, Keiko, YAMAKI, Kohji, TSUSHIDA, Tojiro
Format Journal Article
LanguageEnglish
Published Tsukuba Japanese Society for Food Science and Technology 2001
Japan Science and Technology Agency
Subjects
3T3-L1 adipocyte
differentiation
flavonoid
glycerol-3-phospate dehydrogenase
lipid accumulation
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Abstract The effect of dietary flavonoids (flavone, isoflavone, flavanone, chalcone, flavonol, and flavonol derivatives) on the differentiation of 3T3-L1 adipocytes was investigated. Glycerol-3-phosphate dehydrogenase (GPDH) activity, which is a hallmark of 3T3-L1 differentiation, was measured. The GPDH activity in the cells treated with quercetin, kaempferol, and isorhamnetin was significantly lower than that in the control cells. A low lipid accumulation in cells stained with Oil-Red O was observed following treatment of these flavonols. The anti-differentiation action of quercetin was more potent than that of its derivatives. Quercetin directly inhibited the activity of GPDH extracted from mature 3T3-L1 adipocytes. The inhibitory action of quercetin on the differentiation of 3T3-L1 cells may involve the direct inhibition of GPDH, which is a key enzyme lipid synthesis.
AbstractList The effect of dietary flavonoids (flavone, isoflavone, flavanone, chalcone, flavonol, and flavonol derivatives) on the differentiation of 3T3-L1 adipocytes was investigated. Glycerol-3-phosphate dehydrogenase (GPDH) activity, which is a hallmark of 3T3-L1 differentiation, was measured. The GPDH activity in the cells treated with quercetin, kaempferol, and isorhamnetin was significantly lower than that in the control cells. A low lipid accumulation in cells stained with Oil-Red O was observed following treatment of these flavonols. The anti-differentiation action of quercetin was more potent than that of its derivatives. Quercetin directly inhibited the activity of GPDH extracted from mature 3T3-L1 adipocytes. The inhibitory action of quercetin on the differentiation of 3T3-L1 cells may involve the direct inhibition of GPDH, which is a key enzyme lipid synthesis.
Author IWASHITA, Keiko
YAMAKI, Kohji
TSUSHIDA, Tojiro
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  fullname: TSUSHIDA, Tojiro
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SubjectTerms 3T3-L1 adipocyte
differentiation
flavonoid
glycerol-3-phospate dehydrogenase
lipid accumulation
Title Effect of Flavonoids on the Differentiation of 3T3-L1 Adipocytes
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