Three-Dimensional Reconstruction of the Rat Brain Cortical Microcirculation In Vivo

• Published in: Journal of cerebral blood flow and metabolism Vol. 11; no. 3; pp. 353 - 360
• Main Authors: Dirnagl, Ulrich, Villringer, Arno, Gebhardt, Roland, Haberl, Roman L., Schmiedek, Peter, Einhäupl, Karl M.
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.05.1991; Lippincott Williams & Wilkins
• Subjects: Animals; Biological and medical sciences; Capillaries - anatomy & histology; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges; Cerebral Cortex - blood supply; Fundamental and applied biological sciences. Psychology; Image Processing, Computer-Assisted; Male; Microcirculation - anatomy & histology; Microscopy - methods; Microscopy, Fluorescence; Parietal Lobe - blood supply; Pia Mater - blood supply; Rats; Rats, Inbred Strains; Subarachnoid Space - blood supply; Vertebrates: nervous system and sense organs; Cranial window; Fluorescein angiography; Optical sectioning; Cerebral microcirculation; Confocal laser scanning microscopy; Regional blood flow; Brain; Cerebral cortex; Angiography; Rat; Rodentia; Central nervous system; Laser microscope; Vertebrata; Mammalia; Three dimensional representation; Morphology; Hemodynamics

We used confocal laser scanning microscopy (CLSM) to investigate the morphology and three-dimensional relationships of the microcirculation of the superficial layers of the rat brain cortex in vivo. In anesthetized rats equipped with a closed cranial window (dura mater removed), after i.v. injection...