The Mechanical Stress–Activated Serum-, Glucocorticoid-Regulated Kinase 1 Contributes to Neointima Formation in Vein Grafts

• Published in: Circulation research Vol. 107; no. 10; pp. 1265 - 1274
• Main Authors: Cheng, Jizhong, Wang, Ying, Ma, Yewei, Chan, Bonita Tak-yee, Yang, Min, Liang, Anlin, Zhang, Liping, Li, Huihua, Du, Jie
• Format: Journal Article
• Language: English
• Published: Hagerstown, MD American Heart Association, Inc 12.11.2010; Lippincott Williams & Wilkins
• Subjects: Animals; Biological and medical sciences; Carotid Artery Injuries - enzymology; Carotid Artery Injuries - pathology; Cell Proliferation - drug effects; Cyclin-Dependent Kinase Inhibitor p27 - metabolism; Disease Models, Animal; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling - methods; Gene Expression Regulation, Enzymologic; Immediate-Early Proteins - deficiency; Immediate-Early Proteins - genetics; Immediate-Early Proteins - metabolism; Male; MAP Kinase Kinase 1 - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - enzymology; Muscle, Smooth, Vascular - pathology; Myocytes, Smooth Muscle - drug effects; Myocytes, Smooth Muscle - enzymology; Myocytes, Smooth Muscle - pathology; Oligonucleotide Array Sequence Analysis; Phosphatidylinositol 3-Kinases - metabolism; Phosphorylation; Protein Kinase Inhibitors - pharmacology; Protein Transport; Protein-Serine-Threonine Kinases - deficiency; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Receptor, IGF Type 1 - metabolism; RNA, Messenger - metabolism; Signal Transduction - drug effects; Stress, Mechanical; Time Factors; Tissue Culture Techniques; Trans-Activators - metabolism; Transcription Factors; Transfection; Tunica Intima - drug effects; Tunica Intima - enzymology; Tunica Intima - pathology; Veins - enzymology; Veins - pathology; Veins - transplantation; Vertebrates: cardiovascular system; neointima formation; Sirolimus; Enzyme; Transferases; Mitogen-activated protein kinase; Lactone; mechanical stretch; Macrolide; Glucocorticoid; Macrocycle; Vertebrata; Mammalia; Mechanical stress; Kinase; Blood vessel; serum-, glucocorticoid-regulated kinase-1; mammalian target of rapamycin complex; Graft; Protein synthesis inhibitor; Circulatory system; Vein; Formation; mTORC

RATIONALE:Mechanical stress plays an important role in proliferation of venous smooth muscle cells (SMCs) in neointima, a process of formation that contributes to failure of vein grafts. However, it is unknown what intracellular growth signal leads to proliferation of venous SMCs. OBJECTIVE:The obje...