Increased Bacterial Hemolytic Activity is Conferred by Expression of TlyA Methyltransferase but not by its 2′-O-methylation of the Ribosome

• Published in: Current microbiology Vol. 67; no. 1; pp. 61 - 68
• Main Author: Monshupanee, Tanakarn
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.07.2013; Springer Nature B.V
• Subjects: Antibiotics; Bacteria; Bacteriology; Biomedical and Life Sciences; Biotechnology; Cloning, Molecular; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression; Hemolysin Proteins - genetics; Hemolysin Proteins - metabolism; hemolysis; Life Sciences; Methylation; Methyltransferases - genetics; Methyltransferases - metabolism; Microbiology; Mycobacterium - enzymology; Mycobacterium - genetics; Mycobacterium tuberculosis; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; ribosomal RNA; ribosomes; RNA, Ribosomal - metabolism; Bacterial Ribosome; Tuberculosis; Primer Extension Analysis; Hemolytic Activity; Capreomycin

Bacterial 2′-O-methyltransferase TlyA methylates either both nucleotide C1409 of 16S rRNA and C1920 of 23S rRNA or only the C1920. Both ribosomal methylations increase bacterial susceptibility to ribosome-targeting antibiotics capreomycin and viomycin. However, TlyA has been suggested to also functi...