Rosenbergiella australoborealis sp. nov., Rosenbergiella collisarenosi sp. nov. and Rosenbergiella epipactidis sp. nov., three novel bacterial species isolated from floral nectar

• Published in: Systematic and applied microbiology Vol. 37; no. 6; pp. 402 - 411
• Main Authors: Lenaerts, Marijke, Álvarez-Pérez, Sergio, de Vega, Clara, Van Assche, Ado, Johnson, Steven D., Willems, Kris A., Herrera, Carlos M., Jacquemyn, Hans, Lievens, Bart
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2014
• Subjects: Base Composition; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Enterobacteriaceae; Enterobacteriaceae - classification; Enterobacteriaceae - genetics; Enterobacteriaceae - isolation & purification; Genes, Essential; Molecular Sequence Data; Nectar; Phenotype; Phylogeny; Plant Nectar; RNA, Ribosomal, 16S - genetics; Rosenbergiella; PP; Nectar; MP; Rosenbergiella; SH; BI; MLSA; NJ; ML; Enterobacteriaceae
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2014.03.002

The taxonomic status of nine strains of the family Enterobacteriaceae isolated from floral nectar of wild Belgian, French, South African and Spanish insect-pollinated plants was investigated following a polyphasic approach. Confirmation that these strains belonged to the genus Rosenbergiella was obtained from comparative analysis of partial sequences of the 16S rRNA gene and other core housekeeping genes (atpD [ATP synthase β-chain], gyrB [DNA gyrase subunit B] and rpoB [RNA polymerase β-subunit]), DNA–DNA reassociation data, determination of the DNA G+C content and phenotypic profiling. Two strains belonged to the recently described species Rosenbergiella nectarea, while the other seven strains represented three novel species within the genus Rosenbergiella. The names Rosenbergiella australoborealis sp. nov. (with strain CdVSA 20.1T [LMG 27954T=CECT 8500T] as the type strain), Rosenbergiella collisarenosi sp. nov. (with strain 8.8AT [LMG 27955T=CECT 8501T] as the type strain) and Rosenbergiella epipactidis sp. nov. (with strain 2.1AT [LMG 27956T=CECT 8502T] as the type strain) are proposed. Additionally, the description of the genus Rosenbergiella is updated on the basis of new phenotypic and molecular data.