A Membrane Filter-Assisted Mammalian Cell-Based Biosensor Enabling 3D Culture and Pathogen Detection

• Published in: Sensors (Basel, Switzerland) Vol. 21; no. 9; p. 3042
• Main Authors: Cho, Il-Hoon, Jeon, Jin-Woo, Choi, Min-Ji, Cho, Hyun-Mo, Lee, Jong-Sung, Kim, Dong-Hyung
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 26.04.2021; MDPI
• Subjects: 3D cell culture; Animals; Bacteria; Biosensing Techniques; Biosensors; Cartridges; Cell adhesion & migration; Cell culture; Cell Culture Techniques; Cell growth; cell-based biosensor; Color imagery; Colorimetry; Communication; E coli; Escherichia coli; Ethanol; Gene expression; Hydrophobicity; Immunoassay; Lasers; long-term culture; membrane filter-assisted cell-based biosensor; Membrane filters; Membranes; pathogen detection; Pathogens; Penicillin; Polyesters; Scaffolds; United States > US; long-term culture; membrane filter-assisted cell-based biosensor; 3D cell culture; pathogen detection; cell-based biosensor; immunoassay

We have developed a membrane filter-assisted cell-based biosensing platform by using a polyester membrane as a three-dimensional (3D) cell culture scaffold in which cells can be grown by physical attachment. The membrane was simply treated with ethanol to increase surficial hydrophobicity, inducing...