Multi-scale mechanical characterization of prostate cancer cell lines: Relevant biological markers to evaluate the cell metastatic potential

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 12; pp. 3109 - 3119
• Main Authors: Zouaoui, J., Trunfio-Sfarghiu, A.M., Brizuela, L., Piednoir, A., Maniti, O., Munteanu, B., Mebarek, S., Girard-Egrot, A., Landoulsi, A., Granjon, T.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2017
• Subjects: Atomic force microscopy; Biomarkers; cell aggregates; Cell Line, Tumor; cell lines; Cell membrane; Cell viscoelasticity; cytoskeleton; Cytoskeleton - physiology; deformation; Diffusion; diffusivity; Elasticity; Fluorescence Recovery After Photobleaching; FRAP; Humans; lipids; Male; metastasis; Microscopy, Atomic Force; Middle Aged; neoplasm cells; Neoplasm Metastasis; neoplasms; Prostate cancer; Prostatic Neoplasms - pathology; Rheology; rheometers; stress relaxation; Stress, Mechanical; viscoelasticity; Viscosity; Cell viscoelasticity; Atomic force microscopy; FRAP; Cell membrane; Rheology; Prostate cancer

Considering the importance of cellular mechanics in the birth and evolution of cancer towards increasingly aggressive stages, we compared nano-mechanical properties of non-tumoral (WPMY-1) and highly aggressive metastatic (PC-3) prostate cell lines both on cell aggregates, single cells, and membrane...