Recombinant protein-based ELISA for detection and differentiation of antibodies against avian reovirus in vaccinated and non-vaccinated chickens

• Published in: Journal of virological methods Vol. 165; no. 1; pp. 108 - 111
• Main Authors: Xie, Zhixun, Qin, Chunxiang, Xie, Liji, Liu, Jiabo, Pang, Yaoshan, Deng, Xianwen, Xie, Zhiqin, Khan, Mazhar I.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.04.2010; Elsevier
• Subjects: Agar; Animals; Antibodies, Viral - blood; Antigens, Viral - genetics; Antigens, Viral - isolation & purification; ARV; Avian reovirus; Biological and medical sciences; Chickens; Clinical Laboratory Techniques - methods; Cloning, Molecular; Enzyme-Linked Immunosorbent Assay - methods; Fundamental and applied biological sciences. Psychology; Gene Expression; Indirect ELISA; Microbiology; Nonstructural proteins; Orthoreovirus, Avian - immunology; Plasmids; Poultry Diseases - diagnosis; Recombinant Proteins - genetics; Recombinant Proteins - isolation & purification; Reoviridae Infections - diagnosis; Reoviridae Infections - immunology; Reoviridae Infections - veterinary; Sensitivity and Specificity; Techniques used in virology; Viral Vaccines - immunology; Virology; ARV; Indirect ELISA; Nonstructural proteins; Antibody; Vaccine; Method; Avian reovirus; Reoviridae; Virus; Vertebrata; Orthoreovirus; Chicken; Recombinant protein; Aves; Detection; Veterinary; ELISA assay

Two nonstructural genes, σNS and P17, of avian reovirus (ARV) were cloned into the expression plasmid vector PGEX4T-1. Expressed proteins for σNS and P17 of avian reovirus were purified and used as antigens. Three indirect σNS enzyme-linked immunosorbent assays (ELISAs), σNS-ELISA, P17-ELISA and σNS...