Comparison of gene expression in human periodontal ligament cells cultured from teeth immediately after extraction and from teeth cryopreserved for 1 week

• Published in: Cryobiology Vol. 60; no. 3; pp. 326 - 330
• Main Authors: Min, Kyung-San, Lee, Hyeon-Woo, Lee, Hee-Su, Lee, Jeong-Ho, Park, Sang Hyuk
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Inc 01.06.2010; Elsevier
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; cDNA microarray; Clinical death. Palliative care. Organ gift and preservation; Cryopreservation; Diverse techniques; Fibroblast growth factor receptor 2; Fundamental and applied biological sciences. Psychology; Gene Expression Profiling; Humans; Medical sciences; Molecular and cellular biology; Oligonucleotide Array Sequence Analysis; Periodontal Ligament - cytology; Periodontal ligament cell; Quantitative real-time PCR; Receptor, Fibroblast Growth Factor, Type 2 - biosynthesis; Receptor, Fibroblast Growth Factor, Type 2 - genetics; Reverse Transcriptase Polymerase Chain Reaction; RT-PCR; Tooth - physiology; Periodontal ligament cell; Quantitative real-time PCR; cDNA microarray; Cryopreservation; RT-PCR; Fibroblast growth factor receptor 2; Human; Cell culture; Teeth; DNA chip; Gene expression; Cryobiology; Polymerase chain reaction; Periodontal ligament; Organ preservation; Young adult; Tooth; Molecular biology; Reverse transcription polymerase chain reaction; Comparative study

Gene expression was compared by cDNA microarray analysis in human periodontal ligament (PDL) cells cultured from teeth immediately after extraction and from teeth cryopreserved for 1 week. Twenty healthy collateral premolar teeth without caries and restorations were obtained from 10 young patients,...