Folic acid-capped PEGylated magnetic nanoparticles enter cancer cells mostly via clathrin-dependent endocytosis

• Published in: Biochimica et biophysica acta. General subjects Vol. 1861; no. 6; pp. 1578 - 1586
• Main Authors: Allard-Vannier, Emilie, Hervé-Aubert, Katel, Kaaki, Karine, Blondy, Thibaut, Shebanova, Anastasia, Shaitan, Konstantin V., Ignatova, Anastasia A., Saboungi, Marie-Louise, Feofanov, Alexey V., Chourpa, Igor
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2017; Elsevier
• Subjects: Analytical chemistry; Antibiotics, Antineoplastic - chemistry; Antibiotics, Antineoplastic - metabolism; Antibiotics, Antineoplastic - pharmacology; Bioengineering; Biomaterials; Breast Neoplasms - drug therapy; Breast Neoplasms - metabolism; Cancer; Caveolae - metabolism; Caveolae-dependent endocytosis (CvDE); cell membranes; chemical bonding; chemical elements; Chemical Sciences; Clathrin - metabolism; Clathrin-Coated Vesicles - metabolism; Clathrin-dependent endocytosis (CDE); confocal laser scanning microscopy; Confocal laser-scanning microscopy (CLSM); doxorubicin; Doxorubicin - chemistry; Doxorubicin - metabolism; Doxorubicin - pharmacology; Drug Carriers; Electron energy loss spectroscopy (EELS); Endocytosis; Endosomes - metabolism; Female; fluorescence emission spectroscopy; fluorescent dyes; folic acid; Folic acid (FA); Folic Acid - chemistry; Folic Acid - metabolism; HeLa Cells; High angle annular dark field scanning transmission electron microscopy (HAADF-STEM); Humans; hydrodynamics; Imaging; iron oxides; Life Sciences; lysosomes; Lysosomes - metabolism; Magnetics - methods; Magnetite Nanoparticles - chemistry; MCF-7 Cells; Microscopy, Confocal; Microscopy, Electron, Scanning Transmission; Microscopy, Fluorescence, Multiphoton; Nanomedicine - methods; nanoparticles; neoplasm cells; neoplasms; optical properties; polyethylene glycol; Polyethylene Glycols - chemistry; Spectroscopy, Electron Energy-Loss; Superparamagnetic iron oxide nanoparticles (SPIONs); transmission electron microscopy; Two-photon excited (TPE) fluorescence; Uterine Cervical Neoplasms - drug therapy; Uterine Cervical Neoplasms - metabolism; zeta potential; Folic acid (FA); Confocal laser-scanning microscopy (CLSM); High angle annular dark field scanning transmission electron microscopy (HAADF-STEM); Superparamagnetic iron oxide nanoparticles (SPIONs); Clathrin-dependent endocytosis (CDE); Caveolae-dependent endocytosis (CvDE); Two-photon excited (TPE) fluorescence; Electron energy loss spectroscopy (EELS)

This work is focused on mechanisms of uptake in cancer cells of rationally designed, covalently assembled nanoparticles, made of superparamagnetic iron oxide nanoparticles (SPIONs), fluorophores (doxorubicin or Nile Blue), polyethylene glycol (PEG) and folic acid (FA), referred hereinafter as SFP-FA...