Cooperative effects on the compaction of DNA fragments by the nucleoid protein H-NS and the crowding agent PEG probed by Magnetic Tweezers

• Published in: Biochimica et biophysica acta. General subjects Vol. 1864; no. 12; p. 129725
• Main Authors: Cristofalo, M., Marrano, C.A., Salerno, D., Corti, R., Cassina, V., Mammola, A., Gherardi, M., Sclavi, B., Cosentino Lagomarsino, M., Mantegazza, F.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2020; Elsevier
• Subjects: Bacteriology; Biochemistry; Biochemistry, Molecular Biology; Biophysics; DNA; DNA fragmentation; DNA, Bacterial - chemistry; DNA, Bacterial - metabolism; Escherichia coli; Escherichia coli - chemistry; Escherichia coli - metabolism; Escherichia coli Proteins - metabolism; Fimbriae Proteins - metabolism; Force spectroscopy; genome; Genomics; H-NS; Life Sciences; Magnetic Phenomena; Magnetic Tweezers; magnetism; Microbiology and Parasitology; Molecular biology; Molecular Networks; Nucleic Acid Conformation; Nucleoid-associated proteins; Polyethylene Glycols - metabolism; Single molecule; Structural Biology; H-NS; Force spectroscopy; Magnetic Tweezers; Nucleoid-associated proteins; Single molecule

DNA bridging promoted by the H-NS protein, combined with the compaction induced by cellular crowding, plays a major role in the structuring of the E. coli genome. However, only few studies consider the effects of the physical interplay of these two factors in a controlled environment. We apply a sin...