A mutation that causes lability of the androgen receptor under conditions that normally promote transformation to the DNA-binding state

• Published in: The Journal of clinical investigation Vol. 73; no. 4; pp. 1095 - 1104
• Main Authors: Kovacs, W J, Griffin, J E, Weaver, D D, Carlson, B R, Wilson, J D
• Format: Journal Article
• Language: English
• Published: Ann Arbor, MI American Society for Clinical Investigation 01.04.1984
• Subjects: Biological and medical sciences; Child, Preschool; Cytosol - metabolism; Dihydrotestosterone - metabolism; Fibroblasts - metabolism; Gynecology. Andrology. Obstetrics; Hot Temperature; Humans; Hypospadias - genetics; Hypospadias - metabolism; Infant; Male; Male genital diseases; Medical sciences; Mutation; Non tumoral diseases; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Receptors, Cell Surface - metabolism; Receptors, Steroid - genetics; Receptors, Steroid - metabolism; Testosterone - metabolism; Endocrinopathy; Human; Target tissue resistance; Fixation; Androgen; DNA; Hormone receptor complex; Male genital system pathology; Instability; Hormonal receptor

Dihydrotestosterone-receptor complexes formed in human fibroblast cytosol prepared at 0 degrees C in the presence of sodium molybdate can be readily transformed to the DNA-binding state by heating at 25 degrees C. Under these conditions 50-70% of dihydrotestosterone-receptor complexes bind to DNA. W...