Positive Autoregulation of mrkHI by the Cyclic Di-GMP-Dependent MrkH Protein in the Biofilm Regulatory Circuit of Klebsiella pneumoniae

• Published in: Journal of bacteriology Vol. 197; no. 9; pp. 1659 - 1667
• Main Authors: Tan, Jason W. H, Wilksch, Jonathan J, Hocking, Dianna M, Wang, Nancy, Srikhanta, Yogitha N, Tauschek, Marija, Lithgow, Trevor, Robins-Browne, Roy M, Yang, Ji, Strugnell, Richard A
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.05.2015
• Subjects: autoregulation; bacteria; Bacterial Adhesion; Bacteriology; Binding Sites; biofilm; Biofilms; Biofilms - growth & development; biosynthesis; Cyclic GMP - analogs & derivatives; Cyclic GMP - metabolism; Deoxyribonucleic acid; DNA; DNA Mutational Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA-directed RNA polymerase; DNA-Directed RNA Polymerases - metabolism; Electrophoretic Mobility Shift Assay; fimbriae; Fimbriae, Bacterial - physiology; gel electrophoresis; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Bacterial; Gram-negative bacteria; Homeostasis; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; Klebsiella pneumoniae - physiology; microbial colonization; Nosocomial infections; Operon; pathogens; promoter regions; Promoter Regions, Genetic; Protein Binding; Proteins; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; sequence analysis; Sequence Analysis, DNA; tissues; transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; transcriptional activation

Klebsiella pneumoniae is an important cause of nosocomial infections, primarily through the formation of surface-associated biofilms to promote microbial colonization on host tissues. Expression of type 3 fimbriae by K. pneumoniae facilitates surface adherence, a process strongly activated by the cy...