Identification of Acidic Triterpenoid Saponins from Silene vulgaris Using a Methylation-Based Isolation and LC-MS Analysis Strategy

• Published in: Chemical & pharmaceutical bulletin Vol. 73; no. 3; pp. 179 - 188
• Main Authors: Kikuchi, Takashi, Liu, Danyang, Otsuki, Kouharu, Koike, Kazuo, Li, Wei
• Format: Journal Article
• Language: English
• Published: TOKYO The Pharmaceutical Society of Japan 07.03.2025; Pharmaceutical Soc Japan; Japan Science and Technology Agency
• Subjects: Butanol; Chemistry; Chemistry, Medicinal; Chemistry, Multidisciplinary; Chromatography; Chromatography, High Pressure Liquid; Column chromatography; Diazomethane; LC-MS; Life Sciences & Biomedicine; Liquid Chromatography-Mass Spectrometry; Methylation; Molecular Structure; NMR; Nuclear magnetic resonance; Oleanane; Pharmacology & Pharmacy; Physical Sciences; Plant extracts; Saponins; Saponins - chemistry; Saponins - isolation & purification; Science & Technology; Silene; Silene - chemistry; Silene vulgaris; Tandem Mass Spectrometry; Triterpenes - analysis; Triterpenes - chemistry; Triterpenes - isolation & purification; triterpenoid saponin; Oleanane; NMR; LC-MS; WILD; Silene vulgaris; triterpenoid saponin; ROOTS; TRIPLE QUADRUPOLE-TIME

The Silene genus plants in the Caryophyllaceae family are a large genus with over 850 species. It has been known that Silene genus plants contain triterpenoid saponins. These saponins have glucuronic acid in the sugar chain and are difficult to separate in chromatography. In this study, a strategy w...