Evaluation of Sample Preparation Methods from Rice Seeds and Seedlings Suitable for Two-Dimensional Gel Electrophoresis

• Published in: Applied biochemistry and biotechnology Vol. 175; no. 2; pp. 1035 - 1051
• Main Authors: Wongpia, Aphinya, Mahatheeranont, Sugunya, Lomthaisong, Khemika, Niamsup, Hataichanoke
• Format: Journal Article
• Language: English
• Published: Boston Springer-Verlag 01.01.2015; Springer US; Springer Nature B.V
• Subjects: Acetates - chemistry; Acetone; Acetone - chemistry; Agricultural biotechnology; Analytic Sample Preparation Methods - methods; Aquatic plants; Biochemistry; Biotechnology; Buffers; Chemical Precipitation; Chemistry; Chemistry and Materials Science; crop yield; Electrophoresis; Electrophoresis, Gel, Two-Dimensional; Fractionation; molecular weight; Oryza - chemistry; Oryza sativa; phenol; Phenol - chemistry; Phenols; Plant Proteins - analysis; Plant Proteins - chemistry; Plant Proteins - isolation & purification; polyethylene glycol; Polyethylene Glycols - chemistry; proteins; Proteomics; Rice; Sample preparation; Seedlings; Seedlings - chemistry; Seeds; Seeds - chemistry; trichloroacetic acid; Trichloroacetic Acid - chemistry; two-dimensional gel electrophoresis; Rice seedling; Rice seed; Sample preparation; Proteomics; Two-dimensional gel electrophoresis

In a proteomic study, sample preparation is very important because it affects the quality of protein profiles on two-dimensional gel electrophoresis (2-DE). This study investigated the suitability of four protein extraction methods—direct lysis buffer extraction, trichloroacetic acid (TCA)/acetone p...