IPA-3: An Inhibitor of Diadenylate Cyclase of Streptococcus suis with Potent Antimicrobial Activity

Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved i...

Full description

Saved in:
Bibliographic Details
Published inAntibiotics (Basel) Vol. 11; no. 3; p. 418
Main Authors Li, Haotian, Li, Tingting, Zou, Wenjin, Ni, Minghui, Hu, Qiao, Qiu, Xiuxiu, Yao, Zhiming, Fan, Jingyan, Li, Lu, Huang, Qi, Zhou, Rui
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 21.03.2022
MDPI
Subjects
Antibiotics
antimicrobial
Antimicrobial activity
Antimicrobial agents
Antimicrobial resistance
Bacteria
Binding sites
Biofilms
Chromatography
cyclic diadenylate monophosphate
diadenylate cyclase
Drug resistance
E coli
Enzymatic activity
Gram-positive bacteria
Health risks
High performance liquid chromatography
high-throughput screening
Homeostasis
inhibitor
Inhibitors
IPA-3
Kinases
Liquid chromatography
Meningitis
Molecular docking
Physiology
Proteins
Public health
Screening
Streptococcus infections
Streptococcus suis
Therapeutic targets
Virulence
antimicrobial
diadenylate cyclase
inhibitor
Streptococcus suis
high-throughput screening
IPA-3
cyclic diadenylate monophosphate
Online AccessGet full text

Cover

Abstract Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of Streptococcus suis (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2′-dihydroxy-1,1′-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in vitro in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not E. coli, which is devoid of the enzyme, or Streptococcus mutans, in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.
AbstractList Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of Streptococcus suis (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2'-dihydroxy-1,1'-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in vitro in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not E. coli, which is devoid of the enzyme, or Streptococcus mutans, in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of Streptococcus suis (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2'-dihydroxy-1,1'-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in vitro in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not E. coli, which is devoid of the enzyme, or Streptococcus mutans, in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.
Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of Streptococcus suis (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2′-dihydroxy-1,1′-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in vitro in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not E. coli, which is devoid of the enzyme, or Streptococcus mutans, in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.
Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2'-dihydroxy-1,1'-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not , which is devoid of the enzyme, or , in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.
Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic diadenylate monophosphate (c-di-AMP) has recently been identified as an essential signal molecule for some important bacterial pathogens involved in various bacterial physiological processes, leading to its synthase diadenylate cyclase becoming an attractive antimicrobial drug target. In this study, based on the enzymatic activity of diadenylate cyclase of Streptococcus suis (ssDacA), we established a high-throughput method of screening for ssDacA inhibitors. Primary screening with a compound library containing 1133 compounds identified IPA-3 (2,2′-dihydroxy-1,1′-dinapthyldisulfide) as an ssDacA inhibitor. High-performance liquid chromatography (HPLC) analysis further indicated that IPA-3 could inhibit the production of c-di-AMP by ssDacA in vitro in a dose-dependent manner. Notably, it was demonstrated that IPA-3 could significantly inhibit the growth of several Gram-positive bacteria which harbor an essential diadenylate cyclase but not E. coli , which is devoid of the enzyme, or Streptococcus mutans , in which the diadenylate cyclase is not essential. Additionally, the binding site in ssDacA for IPA-3 was predicted by molecular docking, and contains residues that are relatively conserved in diadenylate cyclase of Gram-positive bacteria. Collectively, our results illustrate the feasibility of ssDacA as an antimicrobial target and consider IPA-3 as a promising starting point for the development of a novel antibacterial.
Author Li, Haotian
Zhou, Rui
Li, Tingting
Huang, Qi
Li, Lu
Qiu, Xiuxiu
Ni, Minghui
Zou, Wenjin
Hu, Qiao
Yao, Zhiming
Fan, Jingyan
AuthorAffiliation 3 International Research Center for Animal Disease (Ministry of Science & Technology of China), Wuhan 430070, China
1 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; lht@webmail.hzau.edu.cn (H.L.); li.tingting@webmail.hzau.edu.cn (T.L.); zouwenjin@webmail.hzau.edu.cn (W.Z.); minghuini@webmail.hzau.edu.cn (M.N.); huqiao@webmail.hzau.edu.cn (Q.H.); xiuxiuq@webmail.hzau.edu.cn (X.Q.); yaozhiming@webmail.hzau.edu.cn (Z.Y.); fjy6168@webmail.hzau.edu.cn (J.F.); lilu@mail.hzau.edu.cn (L.L.)
2 Cooperative Innovation Center of Sustainable Pig Production, Wuhan 430070, China
4 The HZAU-HVSEN Institute, Wuhan 430042, China
AuthorAffiliation_xml – name: 4 The HZAU-HVSEN Institute, Wuhan 430042, China
– name: 1 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; lht@webmail.hzau.edu.cn (H.L.); li.tingting@webmail.hzau.edu.cn (T.L.); zouwenjin@webmail.hzau.edu.cn (W.Z.); minghuini@webmail.hzau.edu.cn (M.N.); huqiao@webmail.hzau.edu.cn (Q.H.); xiuxiuq@webmail.hzau.edu.cn (X.Q.); yaozhiming@webmail.hzau.edu.cn (Z.Y.); fjy6168@webmail.hzau.edu.cn (J.F.); lilu@mail.hzau.edu.cn (L.L.)
– name: 3 International Research Center for Animal Disease (Ministry of Science & Technology of China), Wuhan 430070, China
– name: 2 Cooperative Innovation Center of Sustainable Pig Production, Wuhan 430070, China
Author_xml – sequence: 1
  givenname: Haotian
  surname: Li
  fullname: Li, Haotian
– sequence: 2
  givenname: Tingting
  surname: Li
  fullname: Li, Tingting
– sequence: 3
  givenname: Wenjin
  surname: Zou
  fullname: Zou, Wenjin
– sequence: 4
  givenname: Minghui
  surname: Ni
  fullname: Ni, Minghui
– sequence: 5
  givenname: Qiao
  surname: Hu
  fullname: Hu, Qiao
– sequence: 6
  givenname: Xiuxiu
  surname: Qiu
  fullname: Qiu, Xiuxiu
– sequence: 7
  givenname: Zhiming
  surname: Yao
  fullname: Yao, Zhiming
– sequence: 8
  givenname: Jingyan
  surname: Fan
  fullname: Fan, Jingyan
– sequence: 9
  givenname: Lu
  surname: Li
  fullname: Li, Lu
– sequence: 10
  givenname: Qi
  surname: Huang
  fullname: Huang, Qi
– sequence: 11
  givenname: Rui
  orcidid: 0000-0002-7903-9965
  surname: Zhou
  fullname: Zhou, Rui
BackLink https://www.ncbi.nlm.nih.gov/pubmed/35326881$$D View this record in MEDLINE/PubMed
BookMark eNp9kt9rFDEQxxep2Fr7Fwiy4EtfTvNrdxMfhOOselCwoD6H2eykl2MvOZNs5f775rxW2iLmJWHmOx--M5mX1ZEPHqvqNSXvOFfkPfjseheyM4lSwomg8ll1wkinZi2X7OjB-7g6S2lNylGUSyJfVMe84ayVkp5UZnk1n_EP9dzXS78qyBxiHWz9ycGAfjdCxnqxMyMk3Ie_54jbHEwwZkp1mlyqf7u8qq9CRp8LJbuNMzH0DsZ6brK7cXn3qnpuYUx4dnefVj8_X_xYfJ1dfvuyXMwvZ0YwlWcMJUHWEegtl0gVUyjZIKXFDgfbgbSWctp2pDXGgCK2pEk_8L5RlDQC-Wm1PHCHAGu9jW4DcacDOP0nEOK1hlgmNqIu7bd9z-hAOhDQDGA7IgcKvRHcQssK6-OBtZ36DQ6mdBdhfAR9nPFupa_DjZZKiEaIAji_A8Twa8KU9cYlg-MIHsOUNGuFIIzSVhXp2yfSdZiiL6PaqxjnTUN4Ub156Oivlfu_LAJ1EJT5pxTRauMyZBf2Bt2oKdH71dH_WJ1Sy5_U3uP_V3ULuh3LoA
CitedBy_id crossref_primary_10_1016_j_tim_2024_08_006
crossref_primary_10_1093_femsml_uqad021
crossref_primary_10_1128_spectrum_02418_23
crossref_primary_10_1093_femsre_fuad025
Cites_doi 10.1038/nrmicro3069
10.1007/s00294-016-0600-8
10.1186/s12917-018-1732-5
10.1111/1462-2920.13123
10.1038/nprot.2010.5
10.1128/AEM.02590-17
10.1111/cbdd.13962
10.1093/femsre/fuaa019
10.1002/pmic.200800007
10.1039/C6CC03439J
10.1002/0471141755.ph0904s44
10.1371/journal.ppat.1002217
10.1016/j.tim.2017.09.003
10.1128/JB.00691-19
10.1128/JB.00045-18
10.1371/journal.pgen.1009092
10.1021/ac403203x
10.1016/S1473-3099(07)70001-4
10.1111/jam.14831
10.1128/JB.00166-18
10.4238/2015.June.18.34
10.1586/14760584.2015.1101349
10.1038/srep25445
10.1038/nmeth.3213
10.1128/mBio.00282-13
10.1292/jvms.16-0514
10.1093/nar/gku316
10.3390/genes8080197
10.1093/femsle/fnab059
10.1128/IAI.00365-21
10.1007/BF00229240
10.1038/embor.2011.77
10.1371/journal.ppat.1007795
10.4155/fmc-2020-0310
10.1039/C2CS35206K
10.1039/C4CC02916J
10.1039/C5CC10446G
10.1016/j.ijmm.2010.04.018
10.1371/journal.pone.0035206
10.1371/journal.pone.0092560
10.14202/vetworld.2017.556-561
10.1074/jbc.M117.818716
10.21775/cimb.032.473
10.1177/108705719900400206
10.1016/j.mib.2019.10.008
10.1016/j.molcel.2008.02.020
10.1093/nar/gkv342
10.1038/ja.2016.138
10.1186/s13058-015-0564-5
10.1038/emi.2015.69
10.20506/rst.31.1.2096
10.1016/j.bcp.2020.113943
10.3390/pathogens10101310
10.1080/10408398.2015.1077192
10.3389/fimmu.2018.00577
10.1128/MRA.01380-18
10.1128/CMR.00030-10
10.1007/s00248-017-0985-z
10.1016/j.medmal.2017.10.001
10.1093/bioinformatics/btaa1049
ContentType Journal Article
Copyright 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2022 by the authors. 2022
Copyright_xml – notice: 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2022 by the authors. 2022
DBID AAYXX
CITATION
NPM
7QL
7T7
8FD
8FE
8FH
ABUWG
AFKRA
AZQEC
BBNVY
BENPR
BHPHI
C1K
CCPQU
DWQXO
FR3
GNUQQ
HCIFZ
LK8
M7P
P64
PHGZM
PHGZT
PIMPY
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
5PM
DOA
DOI 10.3390/antibiotics11030418
DatabaseName CrossRef
PubMed
Bacteriology Abstracts (Microbiology B)
Industrial and Applied Microbiology Abstracts (Microbiology A)
Technology Research Database
ProQuest SciTech Collection
ProQuest Natural Science Collection
ProQuest Central (Alumni Edition)
ProQuest Central UK/Ireland
ProQuest Central Essentials
Biological Science Collection
ProQuest Central
Natural Science Collection
Environmental Sciences and Pollution Management
ProQuest One Community College
ProQuest Central Korea
Engineering Research Database
ProQuest Central Student
SciTech Premium Collection
ProQuest Biological Science Collection
Biological Science Database
Biotechnology and BioEngineering Abstracts
ProQuest Central Premium
ProQuest One Academic (New)
Publicly Available Content Database
ProQuest One Academic Middle East (New)
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Applied & Life Sciences
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
MEDLINE - Academic
PubMed Central (Full Participant titles)
DOAJ Directory of Open Access Journals
DatabaseTitle CrossRef
PubMed
Publicly Available Content Database
ProQuest Central Student
Technology Research Database
ProQuest One Academic Middle East (New)
ProQuest Central Essentials
ProQuest Central (Alumni Edition)
SciTech Premium Collection
ProQuest One Community College
ProQuest Natural Science Collection
ProQuest Central China
Environmental Sciences and Pollution Management
ProQuest Central
ProQuest One Applied & Life Sciences
Natural Science Collection
ProQuest Central Korea
Bacteriology Abstracts (Microbiology B)
Biological Science Collection
Industrial and Applied Microbiology Abstracts (Microbiology A)
ProQuest Central (New)
ProQuest Biological Science Collection
ProQuest One Academic Eastern Edition
Biological Science Database
ProQuest SciTech Collection
Biotechnology and BioEngineering Abstracts
ProQuest One Academic UKI Edition
Engineering Research Database
ProQuest One Academic
ProQuest One Academic (New)
MEDLINE - Academic
DatabaseTitleList MEDLINE - Academic
Publicly Available Content Database

PubMed
CrossRef
Database_xml – sequence: 1
  dbid: DOA
  name: DOAJ - Directory of Open Access Journals
  url: https://www.doaj.org/
  sourceTypes: Open Website
– sequence: 2
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Pharmacy, Therapeutics, & Pharmacology
Public Health
EISSN 2079-6382
ExternalDocumentID oai_doaj_org_article_3266bb21d07a4a5daf708d1abc43fa62
PMC8944544
35326881
10_3390_antibiotics11030418
Genre Journal Article
GrantInformation_xml – fundername: National Key Research and Development Plans of China
  grantid: No. 2021YFD1800401
GroupedDBID 53G
5VS
8FE
8FH
AADQD
AAFWJ
AAHBH
AAYXX
ADBBV
AFKRA
AFPKN
AFZYC
ALMA_UNASSIGNED_HOLDINGS
AOIJS
BBNVY
BCNDV
BENPR
BHPHI
CCPQU
CITATION
ECGQY
GROUPED_DOAJ
HCIFZ
HYE
IAO
IHR
ITC
KQ8
LK8
M48
M7P
MODMG
M~E
OK1
OZF
PGMZT
PHGZM
PHGZT
PIMPY
PROAC
RPM
NPM
7QL
7T7
8FD
ABUWG
AZQEC
C1K
DWQXO
FR3
GNUQQ
P64
PKEHL
PQEST
PQGLB
PQQKQ
PQUKI
PRINS
7X8
5PM
PUEGO
ID FETCH-LOGICAL-c429t-2e80e270abf38e1929e82d88fe7edf7a8ff1316706ccca90fe820bd3b591054e3
IEDL.DBID M48
ISSN 2079-6382
IngestDate Wed Aug 27 01:32:59 EDT 2025
Thu Aug 21 14:10:48 EDT 2025
Fri Jul 11 04:42:02 EDT 2025
Fri Jul 25 12:05:07 EDT 2025
Thu Jan 02 22:55:35 EST 2025
Thu Apr 24 23:00:18 EDT 2025
Tue Jul 01 02:09:54 EDT 2025
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Issue 3
Keywords antimicrobial
diadenylate cyclase
inhibitor
Streptococcus suis
high-throughput screening
IPA-3
cyclic diadenylate monophosphate
Language English
License https://creativecommons.org/licenses/by/4.0
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c429t-2e80e270abf38e1929e82d88fe7edf7a8ff1316706ccca90fe820bd3b591054e3
Notes ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
These authors contributed equally to this study.
ORCID 0000-0002-7903-9965
OpenAccessLink https://www.proquest.com/docview/2642335503?pq-origsite=%requestingapplication%
PMID 35326881
PQID 2642335503
PQPubID 2032437
ParticipantIDs doaj_primary_oai_doaj_org_article_3266bb21d07a4a5daf708d1abc43fa62
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8944544
proquest_miscellaneous_2644021169
proquest_journals_2642335503
pubmed_primary_35326881
crossref_citationtrail_10_3390_antibiotics11030418
crossref_primary_10_3390_antibiotics11030418
ProviderPackageCode CITATION
AAYXX
PublicationCentury 2000
PublicationDate 20220321
PublicationDateYYYYMMDD 2022-03-21
PublicationDate_xml – month: 3
  year: 2022
  text: 20220321
  day: 21
PublicationDecade 2020
PublicationPlace Switzerland
PublicationPlace_xml – name: Switzerland
– name: Basel
PublicationTitle Antibiotics (Basel)
PublicationTitleAlternate Antibiotics (Basel)
PublicationYear 2022
Publisher MDPI AG
MDPI
Publisher_xml – name: MDPI AG
– name: MDPI
References Commichau (ref_50) 2018; 200
Yang (ref_63) 2015; 12
Brinkac (ref_1) 2017; 74
Zhou (ref_16) 2016; 52
Jeong (ref_39) 2018; 7
Roy (ref_62) 2010; 5
ref_19
Zarrella (ref_48) 2020; 202
Zhang (ref_30) 2008; 8
Peng (ref_21) 2017; 33
Zou (ref_41) 2018; 84
Verma (ref_57) 2020; 177
Hutchings (ref_8) 2019; 51
Du (ref_59) 2015; 14
Robert (ref_64) 2014; 42
Kundra (ref_49) 2021; 89
ref_60
Tan (ref_34) 2021; 130
Wexselblatt (ref_47) 2011; 12
Han (ref_28) 2018; 9
ref_22
Ding (ref_40) 2015; 4
Oh (ref_33) 2017; 79
Witte (ref_46) 2013; 4
Pham (ref_23) 2016; 62
ref_29
Ong (ref_56) 2015; 17
Cheng (ref_58) 2016; 18
Lun (ref_27) 2007; 7
Zheng (ref_54) 2014; 50
Corrigan (ref_17) 2013; 11
Culp (ref_11) 2017; 70
Entzeroth (ref_36) 2009; 44
(ref_18) 2008; 1
Witte (ref_42) 2008; 30
ref_35
King (ref_12) 2021; 98
Zhang (ref_55) 1999; 4
Zeden (ref_24) 2018; 293
Li (ref_38) 2010; 300
ref_37
Tan (ref_26) 2019; 32
Commichau (ref_45) 2018; 26
Haas (ref_4) 2018; 48
Croft (ref_2) 2007; 13
Ferri (ref_3) 2017; 57
Yin (ref_20) 2020; 44
Yang (ref_61) 2015; 43
Lown (ref_10) 1983; 55
(ref_14) 2021; 13
Zhou (ref_51) 2014; 86
ref_44
ref_43
Silver (ref_7) 2011; 24
Kalia (ref_15) 2013; 42
Zarrella (ref_25) 2018; 200
Devi (ref_32) 2017; 10
Steenhuis (ref_13) 2021; 368
Segura (ref_31) 2015; 14
Bush (ref_9) 2012; 31
ref_5
Sintim (ref_52) 2016; 52
Sintim (ref_53) 2016; 6
ref_6
References_xml – volume: 11
  start-page: 513
  year: 2013
  ident: ref_17
  article-title: Cyclic di-AMP: Another second messenger enters the fray
  publication-title: Nat. Rev. Microbiol.
  doi: 10.1038/nrmicro3069
– volume: 62
  start-page: 731
  year: 2016
  ident: ref_23
  article-title: Replenishing the cyclic-di-AMP pool: Regulation of diadenylate cyclase activity in bacteria
  publication-title: Curr. Genet.
  doi: 10.1007/s00294-016-0600-8
– ident: ref_35
  doi: 10.1186/s12917-018-1732-5
– volume: 18
  start-page: 904
  year: 2016
  ident: ref_58
  article-title: Regulation of oxidative response and extracellular polysaccharide synthesis by a diadenylate cyclase in Streptococcus mutans
  publication-title: Environ. Microbiol.
  doi: 10.1111/1462-2920.13123
– volume: 1
  start-page: pe39
  year: 2008
  ident: ref_18
  article-title: Great times for small molecules: C-di-AMP, a second messenger candidate in Bacteria and Archaea
  publication-title: Sci. Signal.
– volume: 5
  start-page: 725
  year: 2010
  ident: ref_62
  article-title: I-TASSER: A unified platform for automated protein structure and function prediction
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2010.5
– volume: 33
  start-page: 1369
  year: 2017
  ident: ref_21
  article-title: c-di-AMP regulates bacterial biofilm formation
  publication-title: Sheng Wu Gong Cheng Xue Bao
– volume: 84
  start-page: e02590-17
  year: 2018
  ident: ref_41
  article-title: Effects of Environmental and Management-Associated Factors on Prevalence and Diversity of Streptococcus suis in Clinically Healthy Pig Herds in China and the United Kingdom
  publication-title: Appl. Environ. Microbiol.
  doi: 10.1128/AEM.02590-17
– volume: 98
  start-page: 1038
  year: 2021
  ident: ref_12
  article-title: Evaluation of small molecule kinase inhibitors as novel antimicrobial and antibiofilm agents
  publication-title: Chem. Biol. Drug Des.
  doi: 10.1111/cbdd.13962
– volume: 13
  start-page: Ra103
  year: 2007
  ident: ref_2
  article-title: Update on the antibacterial resistance crisis
  publication-title: Med. Sci. Monit.
– volume: 44
  start-page: 701
  year: 2020
  ident: ref_20
  article-title: A decade of research on the second messenger c-di-AMP
  publication-title: FEMS Microbiol. Rev.
  doi: 10.1093/femsre/fuaa019
– volume: 8
  start-page: 3506
  year: 2008
  ident: ref_30
  article-title: Identification of immunogenic cell wall-associated proteins of Streptococcus suis serotype 2
  publication-title: Proteomics
  doi: 10.1002/pmic.200800007
– volume: 52
  start-page: 9327
  year: 2016
  ident: ref_16
  article-title: Cyclic dinucleotide (c-di-GMP, c-di-AMP, and cGAMP) signalings have come of age to be inhibited by small molecules
  publication-title: Chem. Commun.
  doi: 10.1039/C6CC03439J
– volume: 44
  start-page: 9.4.1
  year: 2009
  ident: ref_36
  article-title: Overview of high-throughput screening
  publication-title: Curr. Protoc. Pharmacol.
  doi: 10.1002/0471141755.ph0904s44
– ident: ref_44
  doi: 10.1371/journal.ppat.1002217
– volume: 26
  start-page: 175
  year: 2018
  ident: ref_45
  article-title: A delicate connection: C-di-AMP affects cell integrity by controlling osmolyte transport
  publication-title: Trends Microbiol.
  doi: 10.1016/j.tim.2017.09.003
– volume: 202
  start-page: e00691-19
  year: 2020
  ident: ref_48
  article-title: Bacterial second messenger cyclic di-AMP modulates the competence state in Streptococcus pneumoniae
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00691-19
– volume: 200
  start-page: e00045-18
  year: 2018
  ident: ref_25
  article-title: Stress suppressor screening leads to detection of regulation of cyclic di-AMP homeostasis by a Trk Family effector protein in Streptococcus pneumoniae
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00045-18
– ident: ref_43
  doi: 10.1371/journal.pgen.1009092
– volume: 86
  start-page: 2412
  year: 2014
  ident: ref_51
  article-title: Unexpected complex formation between coralyne and cyclic diadenosine monophosphate providing a simple fluorescent turn-on assay to detect this bacterial second messenger
  publication-title: Anal. Chem.
  doi: 10.1021/ac403203x
– volume: 7
  start-page: 201
  year: 2007
  ident: ref_27
  article-title: Streptococcus suis: An emerging zoonotic pathogen
  publication-title: Lancet Infect. Dis.
  doi: 10.1016/S1473-3099(07)70001-4
– volume: 130
  start-page: 797
  year: 2021
  ident: ref_34
  article-title: Antimicrobial resistance phenotypes and genotypes of Streptococcus suis isolated from clinically healthy pigs from 2017 to 2019 in Jiangxi Province, China
  publication-title: J. Appl. Microbiol.
  doi: 10.1111/jam.14831
– volume: 200
  start-page: e00166-18
  year: 2018
  ident: ref_50
  article-title: Coping with an essential poison: A genetic suppressor analysis corroborates a key function of c-di-AMP in controlling potassium ion homeostasis in gram-positive bacteria
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.00166-18
– volume: 14
  start-page: 6917
  year: 2015
  ident: ref_59
  article-title: Diadenylate cyclase evaluation of ssDacA (SSU98_1483) in Streptococcus suis serotype 2
  publication-title: Genet Mol. Res.
  doi: 10.4238/2015.June.18.34
– volume: 14
  start-page: 1587
  year: 2015
  ident: ref_31
  article-title: Streptococcus suis vaccines: Candidate antigens and progress
  publication-title: Expert Rev. Vaccines
  doi: 10.1586/14760584.2015.1101349
– volume: 6
  start-page: 25445
  year: 2016
  ident: ref_53
  article-title: Inhibition of cyclic diadenylate cyclase, DisA, by polyphenols
  publication-title: Sci. Rep.
  doi: 10.1038/srep25445
– volume: 12
  start-page: 7
  year: 2015
  ident: ref_63
  article-title: The I-TASSER Suite: Protein structure and function prediction
  publication-title: Nat Methods
  doi: 10.1038/nmeth.3213
– volume: 4
  start-page: e00282-13
  year: 2013
  ident: ref_46
  article-title: Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall homeostasis, and establishment of infection
  publication-title: mBio
  doi: 10.1128/mBio.00282-13
– volume: 79
  start-page: 780
  year: 2017
  ident: ref_33
  article-title: Capsular serotypes, virulence-associated genes and antimicrobial susceptibility of Streptococcus suis isolates from pigs in Korea
  publication-title: J. Vet. Med. Sci.
  doi: 10.1292/jvms.16-0514
– volume: 42
  start-page: W320
  year: 2014
  ident: ref_64
  article-title: Deciphering key features in protein structures with the new ENDscript server
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gku316
– ident: ref_22
  doi: 10.3390/genes8080197
– volume: 368
  start-page: fnab059
  year: 2021
  ident: ref_13
  article-title: A ban on BAM: An update on inhibitors of the β-barrel assembly machinery
  publication-title: FEMS Microbiol. Lett.
  doi: 10.1093/femsle/fnab059
– volume: 89
  start-page: e0036521
  year: 2021
  ident: ref_49
  article-title: c-di-AMP is essential for the virulence of Enterococcus faecalis
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.00365-21
– volume: 55
  start-page: 17
  year: 1983
  ident: ref_10
  article-title: The mechanism of action of quinone antibiotics
  publication-title: Mol. Cell Biochem.
  doi: 10.1007/BF00229240
– volume: 12
  start-page: 594
  year: 2011
  ident: ref_47
  article-title: c-di-AMP reports DNA integrity during sporulation in Bacillus subtilis
  publication-title: EMBO Rep.
  doi: 10.1038/embor.2011.77
– ident: ref_29
  doi: 10.1371/journal.ppat.1007795
– volume: 13
  start-page: 143
  year: 2021
  ident: ref_14
  article-title: Targeting the bacterial SOS response for new antimicrobial agents: Drug targets, molecular mechanisms and inhibitors
  publication-title: Future Med. Chem.
  doi: 10.4155/fmc-2020-0310
– volume: 42
  start-page: 305
  year: 2013
  ident: ref_15
  article-title: Nucleotide, c-di-GMP, c-di-AMP, cGMP, cAMP, (p)ppGpp signaling in bacteria and implications in pathogenesis
  publication-title: Chem. Soc. Rev.
  doi: 10.1039/C2CS35206K
– volume: 50
  start-page: 11234
  year: 2014
  ident: ref_54
  article-title: Identification of bromophenol thiohydantoin as an inhibitor of DisA, a c-di-AMP synthase, from a 1000 compound library, using the coralyne assay
  publication-title: Chem. Commun.
  doi: 10.1039/C4CC02916J
– volume: 52
  start-page: 3754
  year: 2016
  ident: ref_52
  article-title: Potent inhibition of cyclic diadenylate monophosphate cyclase by the antiparasitic drug, suramin
  publication-title: Chem. Commun.
  doi: 10.1039/C5CC10446G
– volume: 300
  start-page: 482
  year: 2010
  ident: ref_38
  article-title: Identification of Streptococcus suis serotype 2 genes preferentially expressed in the natural host
  publication-title: Int. J. Med. Microbiol.
  doi: 10.1016/j.ijmm.2010.04.018
– ident: ref_19
  doi: 10.1371/journal.pone.0035206
– ident: ref_60
  doi: 10.1371/journal.pone.0092560
– volume: 10
  start-page: 556
  year: 2017
  ident: ref_32
  article-title: Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India
  publication-title: Vet. World
  doi: 10.14202/vetworld.2017.556-561
– volume: 293
  start-page: 3180
  year: 2018
  ident: ref_24
  article-title: Cyclic di-adenosine monophosphate (c-di-AMP) is required for osmotic regulation in Staphylococcus aureus but dispensable for viability in anaerobic conditions
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.M117.818716
– volume: 32
  start-page: 473
  year: 2019
  ident: ref_26
  article-title: Recent proceedings on prevalence and pathogenesis of Streptococcus suis
  publication-title: Curr. Issues Mol. Biol.
  doi: 10.21775/cimb.032.473
– ident: ref_6
– volume: 4
  start-page: 67
  year: 1999
  ident: ref_55
  article-title: A simple statistical parameter for use in evaluation and validation of high throughput screening assays
  publication-title: J. Biomol. Screen
  doi: 10.1177/108705719900400206
– volume: 51
  start-page: 72
  year: 2019
  ident: ref_8
  article-title: Antibiotics: Past, present and future
  publication-title: Curr. Opin. Microbiol.
  doi: 10.1016/j.mib.2019.10.008
– volume: 30
  start-page: 167
  year: 2008
  ident: ref_42
  article-title: Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates
  publication-title: Mol. Cell
  doi: 10.1016/j.molcel.2008.02.020
– volume: 43
  start-page: W174
  year: 2015
  ident: ref_61
  article-title: I-TASSER server: New development for protein structure and function predictions
  publication-title: Nucleic Acids Res
  doi: 10.1093/nar/gkv342
– volume: 70
  start-page: 366
  year: 2017
  ident: ref_11
  article-title: Bacterial proteases, untapped antimicrobial drug targets
  publication-title: J. Antibiot.
  doi: 10.1038/ja.2016.138
– volume: 17
  start-page: 59
  year: 2015
  ident: ref_56
  article-title: Small molecule inhibition of group I p21-activated kinases in breast cancer induces apoptosis and potentiates the activity of microtubule stabilizing agents
  publication-title: Breast Cancer Res.
  doi: 10.1186/s13058-015-0564-5
– volume: 4
  start-page: e69
  year: 2015
  ident: ref_40
  article-title: Virulence determinants, antimicrobial susceptibility, and molecular profiles of Erysipelothrix rhusiopathiae strains isolated from China
  publication-title: Emerg. Microbes Infect.
  doi: 10.1038/emi.2015.69
– volume: 31
  start-page: 43
  year: 2012
  ident: ref_9
  article-title: Antimicrobial agents targeting bacterial cell walls and cell membranes
  publication-title: Rev. Sci. Tech.
  doi: 10.20506/rst.31.1.2096
– volume: 177
  start-page: 113943
  year: 2020
  ident: ref_57
  article-title: PAK1 inhibitor IPA-3 mitigates metastatic prostate cancer-induced bone remodeling
  publication-title: Biochem. Pharmacol.
  doi: 10.1016/j.bcp.2020.113943
– ident: ref_5
  doi: 10.3390/pathogens10101310
– volume: 57
  start-page: 2857
  year: 2017
  ident: ref_3
  article-title: Antimicrobial resistance: A global emerging threat to public health systems
  publication-title: Crit. Rev. Food Sci. Nutr.
  doi: 10.1080/10408398.2015.1077192
– volume: 9
  start-page: 577
  year: 2018
  ident: ref_28
  article-title: Triggering receptor expressed on myeloid cells-1 signaling: Protective and pathogenic roles on Streptococcal toxic-shock-like syndrome caused by Streptococcus suis
  publication-title: Front. Immunol.
  doi: 10.3389/fimmu.2018.00577
– volume: 7
  start-page: e01380-18
  year: 2018
  ident: ref_39
  article-title: Complete Genome Sequence of Bacillus subtilis Strain WB800N, an Extracellular Protease-Deficient Derivative of Strain 168
  publication-title: Microbiol. Resour. Announc.
  doi: 10.1128/MRA.01380-18
– volume: 24
  start-page: 71
  year: 2011
  ident: ref_7
  article-title: Challenges of antibacterial discovery
  publication-title: Clin. Microbiol. Rev.
  doi: 10.1128/CMR.00030-10
– volume: 74
  start-page: 1001
  year: 2017
  ident: ref_1
  article-title: The threat of antimicrobial resistance on the human microbiome
  publication-title: Microb. Ecol.
  doi: 10.1007/s00248-017-0985-z
– volume: 48
  start-page: 159
  year: 2018
  ident: ref_4
  article-title: Understanding the virulence of Streptococcus suis: A veterinary, medical, and economic challenge
  publication-title: Med. Mal. Infect.
  doi: 10.1016/j.medmal.2017.10.001
– ident: ref_37
  doi: 10.1093/bioinformatics/btaa1049
SSID ssj0000913808
Score 2.210785
Snippet Antimicrobial resistance (AMR) poses a huge threat to public health. The development of novel antibiotics is an effective strategy to tackle AMR. Cyclic...
SourceID doaj
pubmedcentral
proquest
pubmed
crossref
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
Enrichment Source
StartPage 418
SubjectTerms Antibiotics
antimicrobial
Antimicrobial activity
Antimicrobial agents
Antimicrobial resistance
Bacteria
Binding sites
Biofilms
Chromatography
cyclic diadenylate monophosphate
diadenylate cyclase
Drug resistance
E coli
Enzymatic activity
Gram-positive bacteria
Health risks
High performance liquid chromatography
high-throughput screening
Homeostasis
inhibitor
Inhibitors
IPA-3
Kinases
Liquid chromatography
Meningitis
Molecular docking
Physiology
Proteins
Public health
Screening
Streptococcus infections
Streptococcus suis
Therapeutic targets
Virulence
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELZQT1wQbwIFGQn1tFH9TBxuS6FqOaA9tFJvkZ9qpJJUJHvYf8_YTre7qIIL19hJJp7xzDex_Q1Cn5x01tShLisHKQokELxUgJrLoIOrKinBKSa2zx_V2aX4fiWvdkp9xT1hmR44D9wxwIvKGEYdqbXQ0ulQE-WoNlbwoLP3hZi3k0wlH9xQrojKNEMc8vpjkLMz3RC5j2ksrSVimY-dUJQY-x-CmX_ultwJP6dP0ZMZN-JllvcZeuT75-holYmnNwt8cX-OalzgI7y6p6TevED2fLUs-We87PF5fw0SQqaNh4C_dhr8zuYGACc-2VhA0j5ejkvVt9MAvtKuRzyuuxHH_7V4NQDCnuApU_ezSwxOUSSbC1C8RJen3y5Ozsq5vEJpIQhNJfOKeFYTbQJXHpBe4xVzSgVfexdqrUKg8Zw8qSyouSEBmolx3EiAGFJ4_god9EPv3yCcVjMbw4kTQVAttaZUc6uUN8FJwgrE7ka6tTP3eCyBcdNCDhLV0z6gngIttjfdZuqNv3f_ElW47Rp5s9MFsKZ2tqb2X9ZUoMM7A2jnyTy2gBkZB1xGeIE-bpthGsa1Fd37YZ36QCZOadUU6HW2l60kXMJrlaIFqvcsaU_U_Za-u05U36oRQgrx9n982zv0mMWzG4SXjB6ig-nX2r8HRDWZD2ny_AagLyMM
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Central
  dbid: BENPR
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9QwELagvSAhBOUVKMhIqKeNasd24nBB29Kq5VBFqJV6i_ykkUqyNNnD_nvGSTbbRVWvtpOMMvbMN2P7G4S-WmGNznwWpxZCFAggWCwBNcdeeZumQoBR7Nk-L9KzK_7zWlyPCbd2PFa5tom9obaNCTnyQ3DcCQPnSNj3xd84VI0Ku6tjCY2naBdMsITga_fo5KL4NWVZAuulJHKgG2IQ3x-CvJWumsCBTEOJLR7KfdxzST1z_0Nw8_9Tk_fc0OlL9GLEj3g-KPwVeuLqPXRQDATUqxm-3Nynamf4ABcbaurVHno-ZOnwcPnoNTLnxTxm3_C8xuf1DUgMEThuPP5RKbBHq1sAovh4ZQBhu9ActrAXXQM21Cxb3C6rFoc8Li4aQN4dvKWr_lQ9s1MQ0QyFKd6gq9OTy-OzeCy7EBtwTl2cOElckhGlPZMOEGDuZGKl9C5z1mdKek_D_XmSGlB_Tjx0E22ZFgA9BHfsLdqpm9q9R7jf5cw1I5Z7TpVQilLFjJROeytIEqFk_edLM3KSh9IYtyXEJkFd5QPqitBsemgxUHI8PvwoqHQaGvi0-4bm7nc5Ls8SQGyqdUItyRRXwiqfEWmp0oYzr1IQdH89IcpxkbflZkpG6MvUDcsz7Lmo2jXLfgxE6JSmeYTeDfNnkoQJ-KyUNELZ1szaEnW7p65uegpwmXMuOP_wuFgf0bMk3NYgLE7oPtrp7pbuE2CoTn8eF8o_xy4gLg
  priority: 102
  providerName: ProQuest
Title IPA-3: An Inhibitor of Diadenylate Cyclase of Streptococcus suis with Potent Antimicrobial Activity
URI https://www.ncbi.nlm.nih.gov/pubmed/35326881
https://www.proquest.com/docview/2642335503
https://www.proquest.com/docview/2644021169
https://pubmed.ncbi.nlm.nih.gov/PMC8944544
https://doaj.org/article/3266bb21d07a4a5daf708d1abc43fa62
Volume 11
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fa9swEBZd-7KX0f321gUNRp_iTbIkWx6MkXYt7WDFjAb6ZiRLag2Z3cYOLP_9TraTLiMbe5Vk6ZDuTt9J1ncIvTPCFDpxSRgbCFEggGChBNQcOuVMHAsBTrFj-7yIz6b865W42kGrrKjDBDZbQzufT2o6n73_ebf8DAb_yUecELJ_ABFKXdae1pj6rFmcygdoD7amxFvqtwHvd645pUz2WepIkoage1HPRPS3fjZ2q47UfxsS_fOHyt92qNN99GiAlnjS68JjtGOrJ-gw67mpl2N8ef_UqhnjQ5zds1Yvn6LiPJuE7COeVPi8ugEJIRjHtcNfSgWuaTkDTIqPlwWAbeuL_W32bVuDOy0WDW4WZYP9kS7OagDhLfTSlj_KjuTJi1T0OSqeoenpyeXxWThkYAgL2KfaMLKS2CghSjsmLYDB1MrISOlsYo1LlHSO-qf0JC5AE1LioJpow7QAFCK4Zc_RblVX9iXC3YVnqhkx3HGqhFKUKlZIabUzgkQBilYznRcDPbnPkjHLIUzxy5NvWZ4Ajdcf3fbsHP9ufuSXcN3UU2t3BfX8Oh8sNQc8G2sdUUMSxZUwyiVEGqp0wZlTMQh6sFKAfKWuOcDKiAF0IyxAb9fVYKn--kVVtl50bSBYpzROA_Si15e1JEzAsFLSACUbmrQh6mZNVd50bOAy5Vxw_uo_xn2NHkb-9QZhYUQP0G47X9g3gKlaPUJ7RycX2fdRdyYx6qzmF809Jeg
linkProvider Scholars Portal
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELdG9wASQjC-CgOMBHtqNMd2EgcJoe5LLRtVhTppb8HxxxZpS8qSCvWf4m_knKTtiqa97TV200vufPc7O_c7hD7pQKs0spEXakhRIIFgngDU7FlpdRgGATjFmu1zFA5O-fez4GwD_V3UwrjPKhc-sXbUulBuj3wXAjdlEBwJ-zb97bmuUe50ddFCozGLYzP_Aylb-XV4APr9TOnR4WR_4LVdBTwFvrfyqBHE0IjI1DJhAODERlAthDWR0TaSwlrflYeTUMHTxcTCMEk1SwOIrAE3DO77AG1yBqlMB23uHY7GP5e7Oo5lUxDR0BsxFpNdeD9ZmhWOc9l3Lb24ay9yIwTWnQJug7f_f6V5I-wdPUVPWryK-42BPUMbJt9CO-OG8Hrew5NV_VbZwzt4vKLCnm-hx82uIG6KnZ4jNRz3PfYF93M8zC9AYsj4cWHxQSbB_80vAfji_bkCRG_cZXdkPq0K8NlqVuJylpXY7RvjcQFIv4K7VNlVVjNJORFV0wjjBTq9F4W8RJ28yM1rhOtT1ThlRHPLfRlI6fuSKSFManVAaBfRxZtPVMuB7lpxXCaQCzl1Jbeoq4t6yx9NGwqQu6fvOZUupzr-7vpCcX2etO4gAdAcpin1NYkkl4GWNiJC-zJVnFkZgqDbC4NIWqdSJqsl0EUfl8PgDtwZj8xNMavncIBtfhh30avGfpaSsAD-Vgi_i6I1y1oTdX0kzy5qynERcx5w_uZusT6gh4PJj5PkZDg6foseUVcpQphH_W3Uqa5n5h3gtyp93y4ajH7d9zr9ByWfXbM
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1tb9MwELZGJyEkhGC8FQYYCfZpUR3bSRwkhLp11cpQFaFN2rfg-IVFGklZUqH-NX4d5yRtVzTt277GTnLJnc_P2b7nEPqgA62yyEZeqCFEgQCCeQJQs2el1WEYBOAUG7bPaXh8xr-eB-db6O8yF8Ydq1z6xMZR61K5NfIBTNyUweRI2MB2xyKS0fjL7LfnKki5ndZlOY3WRE7M4g-Eb9XnyQh0_ZHS8dHp4bHXVRjwFPjh2qNGEEMjIjPLhAGwExtBtRDWREbbSAprfZcqTkIFXxoTC80k0ywLYJYNuGHw3HtoO4KoiPTQ9sHRNPm-WuFxjJuCiJbqiLGYDOBf5VleOv5l35X34q7UyLXpsKkacBPU_f_E5rUpcPwYPeqwKx62xvYEbZliB-0lLfn1Yh-frnO5qn28h5M1LfZiBz1sVwhxm_j0FKlJMvTYJzws8KS4AIkh-selxaNcgi9cXAIIxocLBejeuMtu-3xWl-C_1bzC1TyvsFtDxkkJqL-Gp9T5r7xhlXIiqrYoxjN0dicKeY56RVmYlwg3O6xxxojmlvsykNL3JVNCmMzqgNA-oss_n6qOD92V5bhMIS5y6kpvUFcf7a9umrV0ILd3P3AqXXV1XN7NhfLqZ9q5hhQAdJhl1NckklwGWtqICO3LTHFmZQiC7i4NIu0cTJWuh0MfvV81g2tw-z2yMOW86cMBwvlh3EcvWvtZScICeK0Qfh9FG5a1IepmS5FfNPTjIuY84PzV7WK9Q_dhfKbfJtOT1-gBdUkjhHnU30W9-mpu3gCUq7O33ZjB6MddD9N_Jf5h6A
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=IPA-3%3A+An+Inhibitor+of+Diadenylate+Cyclase+of+Streptococcus+suis+with+Potent+Antimicrobial+Activity&rft.jtitle=Antibiotics+%28Basel%29&rft.au=Li%2C+Haotian&rft.au=Li%2C+Tingting&rft.au=Zou%2C+Wenjin&rft.au=Ni%2C+Minghui&rft.date=2022-03-21&rft.issn=2079-6382&rft.eissn=2079-6382&rft.volume=11&rft.issue=3&rft_id=info:doi/10.3390%2Fantibiotics11030418&rft.externalDBID=NO_FULL_TEXT
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=2079-6382&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=2079-6382&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=2079-6382&client=summon