Canine serum C-reactive protein detected by means of a near-patient test for human C-reactive protein

• Published in: Journal of small animal practice Vol. 49; no. 6; pp. 282 - 286
• Main Authors: Kjelgaard-H.ansen, M, Stadler, M, Jensen, A.L
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.06.2008; Blackwell Publishing Ltd
• Subjects: Animals; blood chemistry; blood serum; C-reactive protein; C-Reactive Protein - analysis; chemical concentration; Dog Diseases - blood; Dog Diseases - diagnosis; dogs; Dogs - blood; False Negative Reactions; False Positive Reactions; hematologic tests; Humans; latex agglutination test; Latex Fixation Tests - methods; Latex Fixation Tests - standards; Latex Fixation Tests - veterinary; quantitative analysis; rapid methods; reliability; Sensitivity and Specificity
• DOI: 10.1111/j.1748-5827.2008.00541.x
• ISSN: 0022-4510

The aim of the study was to evaluate the reliability of a rapid human C-reactive protein near-patient slide reversed passive latex agglutination test (Randox) for the semi-quantitative determination of canine serum C-reactive protein. The concentration of C-reactive protein was determined in 244 canine serum samples by an established automated immunoturbidimetric method and in various predilutions by a commercially available reversed passive latex agglutination test for human C-reactive protein. The results were compared to assess if the reversed passive latex agglutination test reflected the results of the established method with special emphasis on the reversed passive latex agglutination test's ability to identify samples characterised as positive or negative by the established method. The reversed passive latex agglutination test reflected the C-reactive protein concentration in canine serum samples at all the tested predilutions (undiluted, 1:4, 1:8 and 1:16). When applying a predilution of 1:8, the positive and negative analytical predictive values for discriminating between positive and negative samples (according to the established quantitative method) were high (0·94 [0·82 to 0·99] and 0·97 [0·93 to 0·99], respectively). In conclusion, this near-patient test was able to reflect the serum C-reactive protein concentration in canine samples in a reliable and clinically useful manner and could be applicable for general practice for evaluating C-reactive protein levels in canine serum.