Characterization and application of a flow system for in vitro multispecies oral biofilm formation

• Published in: Journal of periodontal research Vol. 49; no. 3; pp. 323 - 332
• Main Authors: Blanc, V., Isabal, S., Sánchez, M. C., Llama-Palacios, A., Herrera, D., Sanz, M., León, R.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.06.2014
• Subjects: Actinomyces - growth & development; Aggregatibacter actinomycetemcomitans - growth & development; Anti-Infective Agents, Local - pharmacology; Bacterial Load - drug effects; Bacteriological Techniques; biofilm model; Biofilms - growth & development; Bioreactors; Cetylpyridinium - pharmacology; chlorhexidine; Chlorhexidine - pharmacology; confocal laser scanning microscopy; Durapatite - chemistry; Fusobacterium nucleatum - growth & development; Humans; In Situ Hybridization, Fluorescence; Microbial Sensitivity Tests; Microbial Viability; Microscopy, Confocal; Microscopy, Electron, Scanning; Mouth - microbiology; Mouthwashes - pharmacology; oral bacteria; Porphyromonas gingivalis - growth & development; Saliva - physiology; Sodium Fluoride - pharmacology; Streptococcus oralis - growth & development; Veillonella - growth & development; oral bacteria; chlorhexidine; biofilm model; confocal laser scanning microscopy

Background and Objective Bacteria in the oral cavity grow in the form of biofilms; these structures are subject to constant saliva or gingival crevicular fluid flow conditions. The aims of this study were: (i) to develop and to characterize an in‐vitro biofilm model with oral bacteria growing under...