Improved method for purification of enterotoxin from Clostridium perfringens type A [Food poisoning]

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Published inApplied and Environmental Microbiology Vol. 39; no. 6; pp. 1120 - 1122
Main Authors Granum, P E, Whitaker, J R
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.06.1980
Subjects
Ammonium Sulfate
Chemical Precipitation
Chromatography, Gel
Clostridium perfringens - analysis
Enterotoxins - isolation & purification
Hot Temperature
Hydrogen-Ion Concentration
Solubility
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Abstract Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to AEM .asm.org, visit: AEM       
AbstractList The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium sulfate, first 40% saturated to concentrate the enterotoxin and then 15% saturated. The two precipitations were followed by gel filtration on Sephadex G-100. The enterotoxin appeared to be homogeneous on 7% polyacrylamide gel electrophoresis after this three-step purification procedure, with a recovery of 56% and a 12.3-fold purification. The solubility properties at different pH values, temperatures, and ammonium sulfate concentrations are also given as basis for the purification procedure.
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Author Whitaker J.R
Granum P.E
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The purification procedure of Clostridium perfringens type A enterotoxin has been improved. The cell sonic extract was precipitated twice with ammonium...
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SubjectTerms Ammonium Sulfate
Chemical Precipitation
Chromatography, Gel
Clostridium perfringens - analysis
Enterotoxins - isolation & purification
Hot Temperature
Hydrogen-Ion Concentration
Solubility
Title Improved method for purification of enterotoxin from Clostridium perfringens type A [Food poisoning]
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