Spatial transcriptomics reveals human cortical layer and area specification

• Published in: Nature (London) Vol. 644; no. 8075; pp. 153 - 163
• Main Authors: Qian, Xuyu, Coleman, Kyle, Jiang, Shunzhou, Kriz, Andrea J., Marciano, Jack H., Luo, Chunyu, Cai, Chunhui, Manam, Monica Devi, Caglayan, Emre, Lai, Abbe, Exposito-Alonso, David, Otani, Aoi, Ghosh, Urmi, Shao, Diane D., Andersen, Rebecca E., Neil, Jennifer E., Johnson, Robert, LeFevre, Alexandra, Hecht, Jonathan L., Micali, Nicola, Sestan, Nenad, Rakic, Pasko, Miller, Michael B., Sun, Liang, Stringer, Carsen, Li, Mingyao, Walsh, Christopher A.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 07.08.2025; Nature Publishing Group
• Subjects: 13; 14; 38; 38/32; 38/39; 45; 631/136/368/2430; 631/378/2571/1696; 631/378/368/2430; 82; 82/51; Brain architecture; Cells; Cerebral cortex; Cerebral Cortex - cytology; Cerebral Cortex - embryology; Cerebral Cortex - metabolism; Deep Learning; Female; Fetus - cytology; Fetus - embryology; Fetuses; Fluorescence; Fluorescence in situ hybridization; Gene Expression Profiling; Gene sequencing; Genes; Gestation; Humanities and Social Sciences; Humans; Hybridization; Hypotheses; In Situ Hybridization, Fluorescence; Integrated approach; Localization; Male; multidisciplinary; Multiplexing; Neurons; Neurons - cytology; Neurons - metabolism; Nuclei (cytology); Robustness; Science; Science (multidisciplinary); Segmentation; Single-Cell Analysis; Specifications; Synaptogenesis; Transcriptome - genetics; Transcriptomics; Visual cortex; Visual Cortex - cytology; Visual Cortex - embryology

The human cerebral cortex is composed of six layers and dozens of areas that are molecularly and structurally distinct 1 , 2 , 3 – 4 . Although single-cell transcriptomic studies have advanced the molecular characterization of human cortical development, a substantial gap exists owing to the loss of...