Complete sequencing of the recombinant granulocyte-colony stimulating factor (filgrastim) and detection of biotinylation by mass spectrometry

• Published in: Amino acids Vol. 38; no. 4; pp. 1043 - 1049
• Main Authors: Ahmed, Kareem Eldin A. M, Chen, Wei-Qiang, John, Julius Paul Pradeep, Kang, Sung Ung, Lubec, Gert
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.04.2010; Springer Vienna; Springer Nature B.V
• Subjects: Amines - analysis; Amines - chemistry; Amino Acid Sequence; Analytical Chemistry; Biochemical Engineering; Biochemistry; Biomedical and Life Sciences; Biotin - analogs & derivatives; Biotin - analysis; Biotin - chemistry; Biotinylation; Chromatography, High Pressure Liquid; Computer programs; Electrophoresis, Gel, Two-Dimensional; Enzymes; Filgrastim; Glutamine - analogs & derivatives; Glutamine - analysis; Glutamine - chemistry; Granulocyte Colony-Stimulating Factor - chemistry; Granulocyte Colony-Stimulating Factor - isolation & purification; Granulocyte Colony-Stimulating Factor - metabolism; Humans; Life Sciences; Mass spectrometry; Medical research; Methionine; Methionine - analogs & derivatives; Methionine - analysis; Methionine - chemistry; Microchemistry - methods; Molecular Sequence Data; Molecular Structure; Molecular Weight; Nanostructure; Neurobiology; Original Article; Oxidation-Reduction; Peptide Fragments - chemistry; Peptide Fragments - isolation & purification; Peptides; Proteins; Proteomics; Recombinant; Recombinant Proteins - chemistry; Sequence Analysis, Protein - methods; Sequencing; Software; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; Mascot; Two-dimensional electrophoresis; Modiro; Filgrastim; PentylamineBiotin; Complete sequencing

Granulocyte-colony stimulating factor stimulates production and antibacterial function of neutrophiles. Therapy using the recombinant protein drug represents a major step forward in oncology. The protein has not been, however, completely sequenced at the protein level and this formed the rationale o...