Sustained phospholipase D activation is associated with keratinocyte differentiation

• Published in: Carcinogenesis (New York) Vol. 20; no. 4; pp. 569 - 576
• Main Authors: Jung, EunMi, Betancourt-Calle, Soraya, Mann-Blakeney, RaShawn, Griner, Richard D., Bollag, Wendy Bollinger
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.04.1999; Oxford Publishing Limited (England)
• Subjects: 12-O-tetradecanoylphorbol-13-acetate; 2-diacylglycerol; Animals; Biological and medical sciences; bovine serum albumin; Bradykinin - pharmacology; BSA; Cell Differentiation - drug effects; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; DAG; Enzyme Activation - drug effects; FBS; fetal bovine serum; Fundamental and applied biological sciences. Psychology; half-maximal inhibitory concentration; IC50; insulin transferrin and selenious acid; ITS; ITS + linoleic acid/BSA; Keratinocytes - cytology; Keratinocytes - drug effects; Keratinocytes - enzymology; MEM; Mice; minimum essential medium; Molecular and cellular biology; PEt; phosphatidic acid; Phosphatidic Acids - biosynthesis; phosphatidylethanol; Phosphatidylethanolamines - biosynthesis; phospholipase D; Phospholipase D - physiology; PKC; PLD; Protein Isoforms - agonists; Protein Isoforms - physiology; protein kinase C; Protein Kinase C - drug effects; Protein Kinase C - physiology; SDS; serum-free keratinocyte medium; SFKM; sn-1; sodium dodecyl sulfate; staurosporine; Staurosporine - pharmacology; Stsp; TCA; Tetradecanoylphorbol Acetate - pharmacology; TPA; Transglutaminases - metabolism; trichloroacetic acid; Protein kinase C; Enzyme; Transferases; Rodentia; Biological marker; Esterases; Phosphoric diester hydrolases; Cell differentiation; In vitro; Phospholipase D; Signal transduction; Vertebrata; Mammalia; Enzymatic activity; Mouse; Animal; Bradykinin; Hydrolases; Keratinocyte; Mechanism of action

Our previous results and data in the literature have suggested a potential role for phospholipase D (PLD) in the regulation of epidermal keratinocyte growth and differentiation. Therefore, we investigated the effect of agents reported to modulate keratinocyte growth and differentiation on PLD activa...