Differentiation of Cherry leaf roll virus isolates from various host plants by immunocapture-reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations

• Published in: Journal of virological methods Vol. 157; no. 2; pp. 147 - 154
• Main Authors: Buchhop, Jutta, von Bargen, Susanne, Büttner, Carmen
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.05.2009; Elsevier
• Subjects: 3' Untranslated Regions - genetics; 3′ Non-coding region; Betula - virology; Biological and medical sciences; Birch; Black elderberry; Cherry leaf roll virus; Cluster Analysis; Fundamental and applied biological sciences. Psychology; Genotype; Juglans; Juglans - virology; Microbiology; Molecular Sequence Data; Nepovirus; Nepovirus - classification; Nepovirus - genetics; Nepovirus - isolation & purification; Phylogeny; Plant Diseases - virology; Polymerase Chain Reaction - methods; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; Prunus - virology; RNA, Viral - genetics; Sequence Analysis, DNA; Techniques used in virology; Virology; Walnut; Black elderberry; Birch; Nepovirus; Walnut; 3′ Non-coding region; Plant pathogen; Microbiology; Genotype; Method; Phylogeny; Comoviridae; Cherry leaf roll virus; Virology; Virus; Restriction fragment length polymorphism; 3' Non-coding region; Isolate; Reverse transcription polymerase chain reaction

A restriction fragment length polymorphism assay (RFLP) was developed to differentiate Cherry leaf roll virus (CLRV) isolates according to phylogenetic clades by examining restriction patterns from partial 3′ non-coding region (NCR) genomic fragments (approx. 420 bp). The 3′ NCR fragment from 43 CLR...