A single-molecule method for the quantitation of microRNA gene expression

• Published in: Nature methods Vol. 3; no. 1; pp. 41 - 46
• Main Authors: Neely, Lori A, Patel, Sonal, Garver, Joanne, Gallo, Michael, Hackett, Maria, McLaughlin, Stephen, Nadel, Mark, Harris, John, Gullans, Steve, Rooke, Jenny
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.01.2006
• Subjects: DNA Probes - chemistry; Gene Expression Profiling - methods; Humans; In Situ Hybridization - methods; MicroRNAs - analysis; MicroRNAs - metabolism; Oligonucleotides - chemistry; Oligonucleotides, Antisense - chemistry; Sensitivity and Specificity

MicroRNAs (miRNA) are short endogenous noncoding RNA molecules that regulate fundamental cellular processes such as cell differentiation, cell proliferation and apoptosis through modulation of gene expression. Critical to understanding the role of miRNAs in this regulation is a method to rapidly and...