10-Hydroxy-2-decenoic acid inhibiting the proliferation of fibroblast-like synoviocytes by PI3K–AKT pathway

• Published in: International immunopharmacology Vol. 28; no. 1; pp. 97 - 104
• Main Authors: Wang, Jianguang, Zhang, Wei, Zou, Hai, Lin, Yaoyao, Lin, Ke, Zhou, Zhonghao, Qiang, Jiaping, Lin, Jialiang, Chuka, Chifundo Martha, Ge, Renshan, Zhao, Shujiang, Yang, Xinyu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2015
• Subjects: 10H2DA; Acetylation; Arthritis, Rheumatoid - pathology; Cell Proliferation - drug effects; Cytokines - metabolism; Dose-Response Relationship, Drug; Fatty Acids, Monounsaturated - pharmacology; Fibroblasts - drug effects; Gene Expression - drug effects; HDACIs; Histone Deacetylases - metabolism; Histones - metabolism; Humans; Microarray; Oncogene Protein v-akt - drug effects; Phosphatidylinositol 3-Kinases - drug effects; PI3K–AKT pathway; Primary Cell Culture; Receptors, Cytokine - drug effects; Receptors, Cytokine - genetics; Rheumatoid arthritis; Signal Transduction - drug effects; Synovial Fluid - cytology; Synovial Fluid - drug effects; CNTFR; TNXB; ITGB5; LAMA2; IL23R; ITGB3; LPAR4; CXCL3; FLS; Microarray; THBS3; TNFSF13B; HDACIs; PDGFD; PDGFC; IL15RA; 10H2DA; LAMB2; PI3K–AKT pathway; ITGA2; INHBB; TNFRSF10A; PPBP; PPP2R3A; COL6A2; Rheumatoid arthritis; COL6A1; ITGA11; IL7R; HDACI

To reveal the mechanism of 10H2DA inhibiting the proliferation of fibroblast-like synoviocytes (FLSs) of RA patients. Cell proliferation, HDAC activity and histone acetylation level of FLS cells treated with 10H2DA were detected by MTT assay, Colorimetric HDAC Activity Assay and Western-blot. Differ...